Industrial Relevance of Trichoderma reesei as an Enzyme Producer.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1048-0_2
Abstract: Trichoderma reesei's potential as a rapid and efficient biomass degrader was first recognized in the 1950s when it was isolated from Army textiles during World War II. The microbe secreted cellulases that were degrading cotton-based… read more here.
Keywords: trichoderma reesei; industrial relevance; biology; reesei ... See more keywords
Open the Pores: Electroporation for the Transformation of Trichoderma reesei.
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DOI: 10.1007/978-1-0716-1048-0_6
Abstract: During the electroporation of T. reesei, linearized exogenous DNA is absorbed into swollen conidia by an electrical impulse. The advantage of this method is that it is less time-consuming, less expensive, and easier to perform… read more here.
Keywords: electroporation transformation; electroporation; open pores; reesei ... See more keywords
An overview of Trichoderma reesei co-cultures for the production of lignocellulolytic enzymes.
Sign Up to like & getrecommendations! Published in 2021 at "Applied microbiology and biotechnology"
DOI: 10.1007/s00253-021-11261-7
Abstract: Biorefineries are core facilities for implementing a sustainable circular bioeconomy. These facilities rely on microbial enzymes to hydrolyze lignocellulosic substrates into fermentable sugars. Fungal co-cultures mimic the process of natural biodegradation and have been shown… read more here.
Keywords: production; enzyme activities; trichoderma reesei; lignocellulolytic enzymes ... See more keywords
CRISPR/Cas9-mediated genome editing in Penicillium oxalicum and Trichoderma reesei using 5S rRNA promoter-driven guide RNAs.
Sign Up to like & getrecommendations! Published in 2020 at "Biotechnology letters"
DOI: 10.1007/s10529-020-03024-7
Abstract: OBJECTIVE To construct convenient CRISPR/Cas9-mediated genome editing systems in industrial enzyme-producing fungi Penicillium oxalicum and Trichoderma reesei. RESULTS Employing the 5S rRNA promoter from Aspergillus niger for guide RNA expression, the β-glucosidase gene bgl2 in… read more here.
Keywords: genome editing; oxalicum; crispr cas9; rrna promoter ... See more keywords
Effects of the Transcription Factor Ace2 from Trichoderma reesei on Cellulase and Hemicellulase Expression in Trichoderma orientalis EU7-22
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DOI: 10.1007/s12010-021-03529-5
Abstract: Trichoderma orientalis (T. orientalis) EU7-22 has a complete cellulase system and shows a remarkable enzyme activity with high potential in the industry. Ace2 is an important transcriptional factor for cellulase and hemicellulase expression in Trichoderma… read more here.
Keywords: orientalis eu7; trichoderma reesei; expression; cellulase ... See more keywords
Co-expression of Beta-Glucosidase and Laccase in Trichoderma reesei by Random Insertion with Enhanced Filter Paper Activity
Sign Up to like & getrecommendations! Published in 2017 at "Molecular Biotechnology"
DOI: 10.1007/s12033-017-0018-7
Abstract: Trichoderma reesei strain Rut-C30 was modified with enhanced beta-glycosidase (BGL) activity to balance the cellulase system and generated laccase (LAC) protein for lignin degradation. Initially, the binary plasmid p1300-w1 was constructed to express T. reesei… read more here.
Keywords: random insertion; trichoderma reesei; beta; reesei ... See more keywords
The diversity of glycosylation of cellobiohydrolase I from Trichoderma reesei determined with mass spectrometry.
Sign Up to like & getrecommendations! Published in 2019 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2018.12.013
Abstract: Cellulases are glycosylated enzymes that have wide applications in fields like biofuels. It has been widely accepted that glycosylation of cellulases impact their performance. Trichoderma reesei is the most important cellulase-producer and cellobiohydrolase I (CBHI)… read more here.
Keywords: cbhi; trichoderma reesei; cellobiohydrolase; glycosylation ... See more keywords
Effective and reusable T. reesei immobilized on SBA-15 for monomeric sugar production from cellulose hydrolysis
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DOI: 10.1016/j.biteb.2019.01.014
Abstract: Abstract The purpose of this work is to improve the method for immobilizing Trichoderma reesei enzyme on various supports, namely, SBA-15, TUD-1, and MCM-48, and to study its hydrolysis efficiency. Among the studied supports, SBA-15… read more here.
Keywords: monomeric sugar; sugar; cellulose hydrolysis; immobilized sba ... See more keywords
Light, stress, sex and carbon - The photoreceptor ENVOY as a central checkpoint in the physiology of Trichoderma reesei.
Sign Up to like & getrecommendations! Published in 2018 at "Fungal biology"
DOI: 10.1016/j.funbio.2017.10.007
Abstract: Trichoderma reesei represents one of the most prolific producers of homologous and heterologous proteins. Discovery of the photoreceptor ENV1 as a regulator of cellulase gene expression initiated analysis of light response pathways and their physiological… read more here.
Keywords: central checkpoint; trichoderma reesei; photoreceptor; physiology ... See more keywords
The Duality of the MAPK Signaling Pathway in the Control of Metabolic Processes and Cellulase Production in Trichoderma reesei
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DOI: 10.1038/s41598-018-33383-1
Abstract: In this study, through global transcriptional analysis by RNA-Sequencing, we identified the main changes in gene expression that occurred in two functional mutants of the MAPK genes tmk1 and tmk2 in Trichoderma reesei during sugarcane… read more here.
Keywords: trichoderma reesei; signaling pathway; mapk signaling; expression ... See more keywords
Dual Regulatory Role of Chromatin Remodeler ISW1 in Coordinating Cellulase and Secondary Metabolite Biosynthesis in Trichoderma reesei
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DOI: 10.1128/mbio.03456-21
Abstract: Microorganisms, including Trichoderma reesei, constantly face the challenge to outcompete other species to ensure efficient colonization in their natural habitat. They achieve this usually by adopting two alternative strategies by either maintaining fast growth on… read more here.
Keywords: biosynthesis; trichoderma reesei; role; chromatin remodeler ... See more keywords