Reference gene identification for normalisation of RT‐qPCR analysis in plasma samples of the rat middle cerebral artery occlusion model
Sign Up to like & getrecommendations! Published in 2022 at "Veterinary Medicine and Science"
DOI: 10.1002/vms3.879
Abstract: Abstract Objective In quantitative reverse transcription‐polymerase chain reaction (RT‐qPCR) studies, the selection and validation of reference genes are crucial for the accurate analysis of MicroRNAs (miRNAs) expression. In this work, the optimal reference genes for… read more here.
Keywords: reference; normalisation; analysis; reference gene ... See more keywords
Reference Gene Validation via RT–qPCR for Human iPSC-Derived Neural Stem Cells and Neural Progenitors
Sign Up to like & getrecommendations! Published in 2019 at "Molecular Neurobiology"
DOI: 10.1007/s12035-019-1538-x
Abstract: Correct selection of the reference gene(s) is the most important step in gene expression analysis. The aims of this study were to identify and evaluate the panel of possible reference genes in neural stem cells… read more here.
Keywords: reference; neural progenitors; reference gene; gene ... See more keywords
Identification of a chicken (Gallus gallus) endogenous reference gene (Actb) and its application in meat adulteration.
Sign Up to like & getrecommendations! Published in 2017 at "Food chemistry"
DOI: 10.1016/j.foodchem.2017.05.038
Abstract: The genes commonly used to determine meat species are mainly mitochondrial, but the copy numbers of such genes are high, meaning they cannot be accurately quantified. In this paper, for the first time, the chromosomal… read more here.
Keywords: gene actb; reference gene; gene; endogenous reference ... See more keywords
Reference gene identification and validation for quantitative real-time PCR studies in developing Xenopus laevis
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-18684-1
Abstract: Reference genes are essential for gene expression analysis when using real-time quantitative PCR (RT-qPCR). Xenopus laevis is a popular amphibian model for studying vertebrate embryogenesis and development. Further, X. laevis is ideal for studying thyroid… read more here.
Keywords: real time; reference; pcr; reference gene ... See more keywords
Identification and validation of potential reference gene for effective dsRNA knockdown analysis in Chilo partellus
Sign Up to like & getrecommendations! Published in 2019 at "Scientific Reports"
DOI: 10.1038/s41598-019-49810-w
Abstract: Chilo partellus is an invasive polyphagous pest that has not been effectively managed with chemical pesticides. To select potential dsRNAs for use in an alternate control strategy, it is crucial to identify and evaluate stable… read more here.
Keywords: stable reference; reference; chilo partellus; reference gene ... See more keywords
Reference Genes for Expression Studies in Human CD8+ Naïve and Effector Memory T Cells under Resting and Activating Conditions
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-66367-1
Abstract: Reverse-transcription quantitative real-time polymerase chain reaction (RT-qPCR) is widely used for mRNA quantification. To accurately measure changing gene transcript levels under different experimental conditions, the use of appropriate reference gene transcripts is instrumental. In T… read more here.
Keywords: reference genes; reference; reference gene; activating conditions ... See more keywords
Selection of a stable reference gene for relative copy number profiling of porcine chromosomal genes using SYBR green qPCR.
Sign Up to like & getrecommendations! Published in 2022 at "Animal biotechnology"
DOI: 10.1080/10495398.2022.2092742
Abstract: Reference gene with stable copy number is essential for normalization in qPCR based copy number assay. Present study aims to identify a suitable reference gene in pigs for qPCR based relative copy number profiling of… read more here.
Keywords: reference gene; number; copy number;
A reference gene set construction using RNA-seq of multiple tissues of Chinese giant salamander, Andrias davidianus
Sign Up to like & getrecommendations! Published in 2017 at "GigaScience"
DOI: 10.1093/gigascience/gix006
Abstract: Abstract Background Chinese giant salamander (CGS) is the largest extant amphibian species in the world. Owing to its evolutionary position and four peculiar phenomenon of life (longevity, starvation tolerance, regenerative ability, and hatch without sunshine),… read more here.
Keywords: gene set; giant salamander; reference gene; gene ... See more keywords
Reference Gene Optimization for Circadian Gene Expression Analysis in Human Adipose Tissue
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Biological Rhythms"
DOI: 10.1177/0748730419883043
Abstract: A hallmark of biology is the cyclical nature of organismal physiology driven by networks of biological, including circadian, rhythms. Unsurprisingly, disruptions of the circadian rhythms through sleep curtailment or shift work have been connected through… read more here.
Keywords: reference; expression; adipose tissue; reference gene ... See more keywords
Selection of appropriate reference genes for quantitative real-time reverse transcription PCR in Betula platyphylla under salt and osmotic stress conditions
Sign Up to like & getrecommendations! Published in 2019 at "PLoS ONE"
DOI: 10.1371/journal.pone.0225926
Abstract: Selecting appropriate reference genes is vital to normalize gene expression analysis in birch (Betula platyphylla) under different abiotic stress conditions using quantitative real-time reverse transcription PCR (qRT-PCR). In this study, 11 candidate birch reference genes… read more here.
Keywords: appropriate reference; reference; stress; reference gene ... See more keywords
HPRT1 Most Suitable Reference Gene for Accurate Normalization of mRNA Expression in Canine Dermal Tissues with Radiation Therapy
Sign Up to like & getrecommendations! Published in 2022 at "Genes"
DOI: 10.3390/genes13111928
Abstract: Reference genes are crucial in molecular biological studies as an internal control for gene re-search as they exhibit consistent expression patterns across many tissue types. In canines, radiation therapy is the most important therapeutic tool… read more here.
Keywords: reference; canine; expression; reference gene ... See more keywords