Identification of optimal reference genes in Bombyx mori (Lepidoptera) for normalization of stress-responsive genes after challenge with pesticides.
Sign Up to like & getrecommendations! Published in 2022 at "Archives of insect biochemistry and physiology"
DOI: 10.1002/arch.21896
Abstract: Pesticides are frequently used to control pests in agriculture due to their ease of use and effectiveness, but their use causes serious economic losses to sericulture when their production overlaps with agriculture. However, no suitable… read more here.
Keywords: reference genes; relatively stable; bmgapdh tubulin; tubulin ... See more keywords
Selection of stable reference genes for quantitative real-time PCR in the Varroa mite, Varroa destructor.
Sign Up to like & getrecommendations! Published in 2022 at "Archives of insect biochemistry and physiology"
DOI: 10.1002/arch.21905
Abstract: To investigate the acaricide toxicity and resistance mechanisms in the Varroa mite, it is essential to understand the genetic responses of Varroa mites to acaricides, which are usually evaluated by transcriptional profiling based on quantitative… read more here.
Keywords: reference; varroa mite; quantitative real; expression ... See more keywords
Transcriptome‐Based Identification of the Optimal Reference CHO Genes for Normalisation of qPCR Data
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology Journal"
DOI: 10.1002/biot.201700259
Abstract: Real‐time quantitative PCR (qPCR) is the standard method for determination of relative changes in mRNA transcript abundance. Analytical accuracy, precision and reliability are critically dependent on the selection of internal control reference genes. In this… read more here.
Keywords: normalisation qpcr; reference; cho; reference genes ... See more keywords
Evaluation of suitable reference genes for normalization of quantitative reverse transcription PCR analyses in Clavibacter michiganensis
Sign Up to like & getrecommendations! Published in 2019 at "MicrobiologyOpen"
DOI: 10.1002/mbo3.928
Abstract: Clavibacter michiganensis, the causal agent of bacterial canker of tomato, is a Gram‐positive bacterium and a model for studying plant diseases. The real‐time quantitative reverse transcription PCR (real‐time qRT‐PCR) assay is widely used to quantify… read more here.
Keywords: reference genes; suitable reference; michiganensis; gene expression ... See more keywords
Identification of reliable reference genes for expression studies in the magnum of laying hens housed in cage and cage‐free systems
Sign Up to like & getrecommendations! Published in 2021 at "Veterinary Medicine and Science"
DOI: 10.1002/vms3.507
Abstract: Abstract Stress factors during poultry production can evoke changes in gene transcription and protein synthesis in the hen oviduct and could affect the internal and external egg quality. Studies of relative gene expression require the… read more here.
Keywords: production; laying hens; reference; expression ... See more keywords
Reference gene identification for normalisation of RT‐qPCR analysis in plasma samples of the rat middle cerebral artery occlusion model
Sign Up to like & getrecommendations! Published in 2022 at "Veterinary Medicine and Science"
DOI: 10.1002/vms3.879
Abstract: Abstract Objective In quantitative reverse transcription‐polymerase chain reaction (RT‐qPCR) studies, the selection and validation of reference genes are crucial for the accurate analysis of MicroRNAs (miRNAs) expression. In this work, the optimal reference genes for… read more here.
Keywords: reference; normalisation; analysis; reference gene ... See more keywords
Identification and validation of new reference genes for accurate quantitative reverse transcriptase-PCR normalization in the Antarctic plant Colobanthus quitensis under abiotic stress conditions
Sign Up to like & getrecommendations! Published in 2021 at "Polar Biology"
DOI: 10.1007/s00300-021-02801-y
Abstract: The Antarctic ecotype of Colobanthus quitensis is a vascular plant highly adapted to the harsh environmental conditions of Maritime Antarctica which is now facing with the rapid local warming experienced in the Antarctic Peninsula during… read more here.
Keywords: reference; stress conditions; pcr; quitensis ... See more keywords
Gene expression studies of reference genes for quantitative real-time PCR: an overview in insects
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology Letters"
DOI: 10.1007/s10529-017-2465-4
Abstract: Whenever gene expression is being examined, it is essential that a normalization process is carried out to eliminate non-biological variations. The use of reference genes, such as glyceraldehyde-3-phosphate dehydrogenase, actin, and ribosomal protein genes, is… read more here.
Keywords: insects; gene; reference genes; gene expression ... See more keywords
Assessment of reference genes for real-time quantitative PCR normalization in Ilex paraguariensis leaves during drought
Sign Up to like & getrecommendations! Published in 2017 at "Biologia Plantarum"
DOI: 10.1007/s10535-017-0754-3
Abstract: Reverse transcription of RNA followed by real-time quantitative PCR (qPCR) is to date the most reliable method for gene expression studies. However, to control the errors introduced along the numerous experimental procedures, it requires a… read more here.
Keywords: real time; ilex paraguariensis; reference; paraguariensis leaves ... See more keywords
Selection of reference genes for miRNA quantitative PCR and its application in miR-34a/Sirtuin-1 mediated energy metabolism in Megalobrama amblycephala
Sign Up to like & getrecommendations! Published in 2019 at "Fish Physiology and Biochemistry"
DOI: 10.1007/s10695-019-00658-x
Abstract: MiRNAs are small, non-coding RNAs that downregulate gene expression at post-transcriptional levels. They have emerged as important regulators involved in metabolism, immunity, and cancer. Real-time quantitative PCR (RT-qPCR) is an effective and main method for… read more here.
Keywords: quantitative pcr; genes mirna; metabolism; reference ... See more keywords
Exploring reliable reference genes for gene expression normalization in Karenia mikimotoi using real-time PCR
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Applied Phycology"
DOI: 10.1007/s10811-019-01961-z
Abstract: The dinoflagellate Karenia mikimotoi is one of the most noxious species that can form harmful algal blooms in coastal areas worldwide, causing serious damage to aquaculture and fisheries. In the present study, twelve commonly reported… read more here.
Keywords: reference genes; expression; gene expression; karenia mikimotoi ... See more keywords