Metabarcoding free‐living marine nematodes using curated 18S and CO1 reference sequence databases for species‐level taxonomic assignments
Sign Up to like & getrecommendations! Published in 2019 at "Ecology and Evolution"
DOI: 10.1002/ece3.4814
Abstract: Abstract High‐throughput sequencing has the potential to describe biological communities with high efficiency yet comprehensive assessment of diversity with species‐level resolution remains one of the most challenging aspects of metabarcoding studies. We investigated the utility… read more here.
Keywords: sequence databases; reference; reference sequence; taxonomic assignments ... See more keywords
Population-scale detection of non-reference sequence variants using colored de Bruijn Graphs.
Sign Up to like & getrecommendations! Published in 2021 at "Bioinformatics"
DOI: 10.1093/bioinformatics/btab749
Abstract: MOTIVATION With the increasing throughput of sequencing technologies, structural variant (SV) detection has become possible across tens of thousands of genomes. Non-reference sequence (NRS) variants have drawn less attention compared to other types of SVs… read more here.
Keywords: nrs variants; detection; reference sequence; using colored ... See more keywords
MegaPath: sensitive and rapid pathogen detection using metagenomic NGS data
Sign Up to like & getrecommendations! Published in 2020 at "BMC Genomics"
DOI: 10.1186/s12864-020-06875-6
Abstract: Background Next-generation sequencing (NGS) enables unbiased detection of pathogens by mapping the sequencing reads of a patient sample to the known reference sequence of bacteria and viruses. However, for a new pathogen without a reference… read more here.
Keywords: megapath sensitive; pathogen; detection; reference sequence ... See more keywords