Engineering an Overflow‐Responsive Regulation System for Balancing Cellular Redox and Optimizing Microbial Production
Sign Up to like & getrecommendations! Published in 2025 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28976
Abstract: Escherichia coli accumulates acetate as a byproduct in fast growth aerobic conditions when using glucose as carbon source. This phenomenon, known as overflow metabolism, has negative impacts on cell growth and protein expression, also causes… read more here.
Keywords: regulation system; cellular redox; regulation; microbial production ... See more keywords
Rewiring Metabolic Flux in Corynebacterium glutamicum Using a CRISPR/dCpf1-Based Bifunctional Regulation System.
Sign Up to like & getrecommendations! Published in 2024 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.3c08529
Abstract: Corynebacterium glutamicum, a microorganism classified as generally recognized as safe for use in the industrial production of food raw materials and additives, has encountered challenges in achieving widespread adoption and popularization as microbial cell factories.… read more here.
Keywords: crispr; regulation system; bifunctional regulation; metabolic flux ... See more keywords
A DNA Bubble-Mediated Gene Regulation System Based on Thrombin-Bound DNA Aptamers.
Sign Up to like & getrecommendations! Published in 2017 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.6b00391
Abstract: We describe here a novel approach to enhance the transcription of a target gene in cell-free systems by symmetrically introducing duplex aptamers upstream to a T7 promoter in both the sense and antisense strands of… read more here.
Keywords: dna bubble; gene; gene regulation; regulation system ... See more keywords
PRIORITY MEASURES OF LEGAL REGULATION OF THE SYSTEM OF S TATE AND MUNICIPAL PROCUREMENTS
Sign Up to like & getrecommendations! Published in 2020 at "Economic Systems"
DOI: 10.29030/2309-2076-2020-13-1-86-94
Abstract: The paper considers innovations in the field of legal regulation of public procurement at the last stage of the reform carried out in the public finance management system. The main attention is paid to filling… read more here.
Keywords: system; measures legal; regulation system; legal regulation ... See more keywords