Multiple-site diversification of regulatory sequences enables inter-species operability of genetic devices.
Sign Up to like & getrecommendations! Published in 2019 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.9b00375
Abstract: The features of the light-responsive cyanobacterial CcaSR regulatory module that determine interoperability of this optogenetic device between Escherichia coli and Pseudomonas putida have been examined. For this, all structural parts (i.e. ho1 and pcyA genes… read more here.
Keywords: sequences enables; site diversification; multiple site; diversification regulatory ... See more keywords
AAV cis-regulatory sequences are correlated with ocular toxicity
Sign Up to like & getrecommendations! Published in 2019 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.1821000116
Abstract: Significance Adeno-associated viral (AAV) gene therapy is becoming an important therapeutic modality, especially for ocular diseases, due to its efficiency of gene delivery and relative lack of pathogenicity. However, AAV sometimes can cause inflammation and… read more here.
Keywords: toxicity; aav cis; regulatory sequences; cis regulatory ... See more keywords
Characterizing Transcriptional Regulatory Sequences in Coronaviruses and Their Role in Recombination
Sign Up to like & getrecommendations! Published in 2020 at "Molecular Biology and Evolution"
DOI: 10.1093/molbev/msaa281
Abstract: Abstract Novel coronaviruses, including SARS-CoV-2, SARS, and MERS, often originate from recombination events. The mechanism of recombination in RNA viruses is template switching. Coronavirus transcription also involves template switching at specific regions, called transcriptional regulatory… read more here.
Keywords: characterizing transcriptional; coronavirus; trs; transcriptional regulatory ... See more keywords
Accurate Identification of Transcription Regulatory Sequences and Genes in Coronaviruses
Sign Up to like & getrecommendations! Published in 2022 at "Molecular Biology and Evolution"
DOI: 10.1093/molbev/msac133
Abstract: Abstract Transcription regulatory sequences (TRSs), which occur upstream of structural and accessory genes as well as the 5′ end of a coronavirus genome, play a critical role in discontinuous transcription in coronaviruses. We introduce two… read more here.
Keywords: trs sites; transcription; identification; coronavirus ... See more keywords
Reciprocal insulation analysis of Hi-C data shows that TADs represent a functionally but not structurally privileged scale in the hierarchical folding of chromosomes.
Sign Up to like & getrecommendations! Published in 2017 at "Genome research"
DOI: 10.1101/gr.212803.116
Abstract: Understanding how regulatory sequences interact in the context of chromosomal architecture is a central challenge in biology. Chromosome conformation capture revealed that mammalian chromosomes possess a rich hierarchy of structural layers, from multi-megabase compartments to… read more here.
Keywords: analysis; privileged scale; reciprocal insulation; insulation ... See more keywords
Enhancer trap lines with GFP driven by smad6b and frizzled1 regulatory sequences for the study of epithelial morphogenesis in the developing zebrafish inner ear.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of anatomy"
DOI: 10.1111/joa.13845
Abstract: Live imaging in the zebrafish embryo using tissue-specific expression of fluorescent proteins can yield important insights into the mechanisms that drive sensory organ morphogenesis and cell differentiation. Morphogenesis of the semicircular canal ducts of the… read more here.
Keywords: morphogenesis; trap lines; inner ear; regulatory sequences ... See more keywords