Engineering Chlamydomonas reinhardtii for Expression of Functionally Active Human Interferon-α
Sign Up to like & getrecommendations! Published in 2018 at "Molecular Biotechnology"
DOI: 10.1007/s12033-018-0143-y
Abstract: Human interferon (IFN) are secreted cytokines that play a major regulatory role in response to various infections. Commercially, IFN-α has been approved to treat many chronic viral diseases as well as a variety of cancers… read more here.
Keywords: human interferon; expression; reinhardtii expression; chlamydomonas reinhardtii ... See more keywords
Exploring intrinsically disordered proteins in Chlamydomonas reinhardtii
Sign Up to like & getrecommendations! Published in 2018 at "Scientific Reports"
DOI: 10.1038/s41598-018-24772-7
Abstract: The content of intrinsically disordered protein (IDP) is related to organism complexity, evolution, and regulation. In the Plantae, despite their high complexity, experimental investigation of IDP content is lacking. We identified by mass spectrometry 682… read more here.
Keywords: exploring intrinsically; chlamydomonas reinhardtii; proteins chlamydomonas; reinhardtii ... See more keywords
Challenges and advances towards the rational design of microalgal synthetic promoters in Chlamydomonas reinhardtii.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of experimental botany"
DOI: 10.1093/jxb/erad100
Abstract: Microalgae hold the enormous potential to provide a safe and sustainable source of high-value compounds, acting as carbon-fixing biofactories that could help to mitigate rapidly progressing climate change. Bioengineering microalgal strains will be key to… read more here.
Keywords: challenges advances; rational design; chlamydomonas reinhardtii; synthetic promoters ... See more keywords
The effects of 5‐azacytidine and cadmium on global 5‐methylcytosine content and secondary metabolites in the freshwater microalgae Chlamydomonas reinhardtii and Scenedesmus quadricauda
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Phycology"
DOI: 10.1111/jpy.12819
Abstract: Epigenetic changes are important mechanisms in the regulation of chromatin structure and gene expression. Cytosine methylation is one of the major epigenetic modifications, mediated by DNA methyltransferases, which transfer methyl groups from S‐adenosyl‐L‐methionine (SAM) to… read more here.
Keywords: effects azacytidine; content; cadmium; quadricauda ... See more keywords
Overexpression of 18S rRNA methyltransferase CrBUD23 enhances biomass and lutein content in Chlamydomonas reinhardtii
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2023.1102098
Abstract: Post-transcriptional modification of nucleic acids including transfer RNA (tRNA), ribosomal RNA (rRNA) and messenger RNA (mRNA) is vital for fine-tunning of mRNA translation. Methylation is one of the most widespread post-transcriptional modifications in both eukaryotes… read more here.
Keywords: 18s rrna; crbud23; reinhardtii; rrna methyltransferase ... See more keywords
Chemical synthesis of left arm of Chlamydomonas reinhardtii mitochondrial genome and in vivo functional analysis
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2022.1064497
Abstract: Chlamydomonas reinhardtii is a photosynthetic eukaryote showing great industrial potential. The synthesis and in vivo function of the artificial C. reinhardtii genome not only promotes the development of synthetic biology technology but also supports industries… read more here.
Keywords: left arm; synthesis; genome; syn ... See more keywords
Combinatorial Engineering Enables Photoautotrophic Growth in High Cell Density Phosphite-Buffered Media to Support Engineered Chlamydomonas reinhardtii Bio-Production Concepts
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2022.885840
Abstract: Chlamydomonas reinhardtii has emerged as a powerful green cell factory for metabolic engineering of sustainable products created from the photosynthetic lifestyle of this microalga. Advances in nuclear genome modification and transgene expression are allowing robust… read more here.
Keywords: cell; high cell; cell density; engineering ... See more keywords
Selectable Markers and Reporter Genes for Engineering the Chloroplast of Chlamydomonas reinhardtii
Sign Up to like & getrecommendations! Published in 2018 at "Biology"
DOI: 10.3390/biology7040046
Abstract: Chlamydomonas reinhardtii is a model alga of increasing interest as a cell factory for the production of valuable compounds, including therapeutic proteins and bioactive metabolites. Expression of foreign genes in the chloroplast is particularly advantageous… read more here.
Keywords: reporter genes; engineering; chloroplast; expression ... See more keywords