RNA Chemical Labeling with Site‐Specific, Relative Quantification by Mass Spectrometry for the Structural Study of a Neomycin‐Sensing Riboswitch Aptamer Domain
Sign Up to like & getrecommendations! Published in 2022 at "Chempluschem"
DOI: 10.1002/cplu.202200256
Abstract: Abstract High‐resolution mass spectrometry was used for the label‐free, direct localization and relative quantification of CMC+‐modifications of a neomycin‐sensing riboswitch aptamer domain in the absence and presence of the aminoglycoside ligands neomycin B, ribostamycin, and… read more here.
Keywords: mass spectrometry; relative quantification; riboswitch; neomycin sensing ... See more keywords
Relative Quantification of Phosphorylated and Glycosylated Peptides from the Same Sample Using Isobaric Chemical Labelling with a Two-Step Enrichment Strategy.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1024-4_14
Abstract: Post-translational modifications (PTMs) are essential for the regulation of all cellular processes. The interplay of various PTMs on a single protein or different proteins comprises a complexity that we are far from understanding in its… read more here.
Keywords: glycosylated peptides; phosphorylated glycosylated; quantification phosphorylated; peptides sample ... See more keywords
Relative Quantification of siRNA Strand Loading into Ago2 for Design of Highly Active siRNAs.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9220-1_4
Abstract: In RNA interference (RNAi), silencing is achieved through the interaction of double-stranded small interfering RNAs (siRNAs) with essential RNAi pathway proteins, including Argonaute 2 (Ago2). Based on these interactions, one strand of the siRNA is… read more here.
Keywords: loading ago2; sirna strand; strand; relative quantification ... See more keywords
qPCR-based relative quantification of the brown algal endophyte Laminarionema elsbetiae in Saccharina latissima: variation and dynamics of host—endophyte interactions
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Applied Phycology"
DOI: 10.1007/s10811-017-1367-0
Abstract: Morphological changes—such as dark spots, twisted stipes and deformed blades—have been observed in wild and cultivated Saccharina latissima. The putative cause for the disease symptoms is the filamentous endophytic brown alga Laminarionema elsbetiae, which is… read more here.
Keywords: host endophyte; latissima; saccharina latissima; relative quantification ... See more keywords
Simultaneous Relative Quantification of Various Polyglycerophospholipids with Isotope-Labeled Methylation by Nanoflow Ultrahigh Performance Liquid Chromatography-Tandem Mass Spectrometry.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.9b00800
Abstract: Herein, we introduce a comprehensive analytical method for the separation and relative quantification of polyglycerophospholipids (PGPLs), including phosphatidylglycerol (PG), bis(monoacylglycero)phosphate (BMP), bis(diacylglycero)phosphate (BDP), Hemi BDP, cardiolipin (CL), monolysocardiolipin (MLCL), and dilysocardiolipin (DLCL), using isotope-labeled methylation… read more here.
Keywords: labeled methylation; bdp; isotope labeled; relative quantification ... See more keywords
A novel RT-QPCR-based assay for the relative quantification of residue specific m6A RNA methylation
Sign Up to like & getrecommendations! Published in 2019 at "Scientific Reports"
DOI: 10.1038/s41598-019-40018-6
Abstract: N6-methyladenosine (m6A) is the most common and abundant RNA modification. Recent studies have shown its importance in the regulation of several biological processes, including the immune response, and different approaches have been developed in order to… read more here.
Keywords: methylation; qpcr based; rna; relative quantification ... See more keywords
Chainy, an universal tool for standardized relative quantification in real-time PCR.
Sign Up to like & getrecommendations! Published in 2017 at "Bioinformatics"
DOI: 10.1093/bioinformatics/btw839
Abstract: Chainy is a cross-platform web tool providing systematic pipelines and steady criteria to process real-time PCR data, including the calculation of efficiencies from raw data by kinetic methods, evaluation of the suitability of multiple references,… read more here.
Keywords: time pcr; relative quantification; real time; tool ... See more keywords
SpliceLauncher: a tool for detection, annotation and relative quantification of alternative junctions from RNAseq data
Sign Up to like & getrecommendations! Published in 2020 at "Bioinformatics"
DOI: 10.1093/bioinformatics/btz784
Abstract: SUMMARY Alternative splicing is an important biological process widely analyzed in molecular diagnostic settings. Indeed, a variant can be pathogenic by splicing alteration and a suspected pathogenic variant (e.g. truncating variant) can be rescued by… read more here.
Keywords: quantification alternative; splicelauncher tool; annotation relative; relative quantification ... See more keywords
Estimating Population Turnover Rates by Relative Quantification Methods Reveals Microbial Dynamics in Marine Sediment
Sign Up to like & getrecommendations! Published in 2017 at "Applied and Environmental Microbiology"
DOI: 10.1128/aem.01443-17
Abstract: ABSTRACT The difficulty involved in quantifying biogeochemically significant microbes in marine sediments limits our ability to assess interspecific interactions, population turnover times, and niches of uncultured taxa. We incubated surface sediments from Cape Lookout Bight,… read more here.
Keywords: turnover; turnover times; relative quantification; methanogenesis ... See more keywords