Site-selective 1H/2H labeling enables artifact-free 1H CPMG relaxation dispersion experiments in aromatic side chains
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Biomolecular Nmr"
DOI: 10.1007/s10858-019-00275-z
Abstract: Aromatic side chains are often key residues in enzyme active sites and protein binding sites, making them attractive probes of protein dynamics on the millisecond timescale. Such dynamic processes can be studied by aromatic 13C… read more here.
Keywords: dispersion experiments; relaxation dispersion; side chains; relaxation ... See more keywords
Direct evidence for (G)O6···H2-N4(C)+ hydrogen bonding in transient G(syn)-C+ and G(syn)-m5C+ Hoogsteen base pairs in duplex DNA from cytosine amino nitrogen off-resonance R1ρ relaxation dispersion measurements.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of magnetic resonance"
DOI: 10.1016/j.jmr.2019.106589
Abstract: NMR relaxation dispersion studies have shown that Watson-Crick G-C and A-T base pairs in duplex DNA exist in dynamic equilibrium with their Hoogsteen counterparts. Hoogsteen base pairs form through concurrent rotation of the purine base… read more here.
Keywords: relaxation dispersion; syn; base pairs; base ... See more keywords
Multiscale nuclear magnetic relaxation dispersion of complex liquids in bulk and confinement.
Sign Up to like & getrecommendations! Published in 2018 at "Progress in nuclear magnetic resonance spectroscopy"
DOI: 10.1016/j.pnmrs.2017.11.001
Abstract: The nuclear magnetic relaxation dispersion (NMRD) technique consists of measurement of the magnetic-field dependence of the longitudinal nuclear-spin-lattice relaxation rate 1/T1. Usually, the acquisition of the NMRD profiles is made using a fast field cycling… read more here.
Keywords: technique; relaxation dispersion; nuclear magnetic; relaxation ... See more keywords
Resolving biomolecular motion and interactions by R2 and R1ρ relaxation dispersion NMR.
Sign Up to like & getrecommendations! Published in 2018 at "Methods"
DOI: 10.1016/j.ymeth.2018.04.026
Abstract: Among the tools of structural biology, NMR spectroscopy is unique in that it not only derives a static three-dimensional structure, but also provides an atomic-level description of the local fluctuations and global dynamics around this… read more here.
Keywords: motion interactions; relaxation dispersion; dispersion; resolving biomolecular ... See more keywords
Quantifying Microsecond Exchange in Large Protein Complexes with Accelerated Relaxation Dispersion Experiments in the Solid State
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DOI: 10.1038/s41598-019-47507-8
Abstract: Solid state NMR is a powerful method to obtain information on the structure and dynamics of protein complexes that, due to solubility and size limitations, cannot be achieved by other methods. Here, we present an… read more here.
Keywords: exchange; solid state; relaxation dispersion; protein complexes ... See more keywords