Real-time analysis of cleavage and religation activity of human topoisomerase 1 based on ternary fluorescence resonance energy transfer DNA substrate.
Sign Up to like & getrecommendations! Published in 2018 at "Archives of biochemistry and biophysics"
DOI: 10.1016/j.abb.2018.02.006
Abstract: Human topoisomerase 1B is a ubiquitous and essential enzyme involved in relaxing the topological state of supercoiled DNA to allow the progression of fundamental DNA metabolism. Its enzymatic catalytic cycle consists of cleavage and religation… read more here.
Keywords: cleavage religation; energy transfer; dna substrate; religation ... See more keywords
The human DNA topoisomerase I mutant Gly717Asp: Higher religation rate is not always associated with camptothecin resistance.
Sign Up to like & getrecommendations! Published in 2019 at "Archives of biochemistry and biophysics"
DOI: 10.1016/j.abb.2019.01.007
Abstract: DNA topoisomerases are key enzyme responsible for modulating the topological state of the DNA by breaking and rejoining of DNA strand. Characterization of a Gly717Asp mutation in the human topoisomerase was performed using several catalytic… read more here.
Keywords: dna; gly717asp; drug; topoisomerase ... See more keywords