Using Site Specific Fluorescent Probes to Examine Replication Fork Destabilization by Regulatory Proteins of the Bacteriophage T4 DNA Replication Complex
Sign Up to like & getrecommendations! Published in 2017 at "Biophysical Journal"
DOI: 10.1016/j.bpj.2016.11.1705
Abstract: The reconstituted T4 DNA replication complex serves as the simplest model system to examine the ‘core’ replication mechanisms of higher organisms. In this study we used site-specifically introduced fluorescent base analogues to track local conformational… read more here.
Keywords: replication fork; regulatory proteins; dna replication; replication complex ... See more keywords
Physical interactions between MCM and Rad51 facilitate replication fork lesion bypass and ssDNA gap filling by non-recombinogenic functions.
Sign Up to like & getrecommendations! Published in 2021 at "Cell reports"
DOI: 10.1016/j.celrep.2021.109440
Abstract: The minichromosome maintenance (MCM) helicase physically interacts with the recombination proteins Rad51 and Rad52 from yeast to human cells. We show, in Saccharomyces cerevisiae, that these interactions occur within a nuclease-insoluble scaffold enriched in replication/repair… read more here.
Keywords: replication fork; ssdna gap; mcm rad51; non recombinogenic ... See more keywords
Regulation of replication fork speed: Mechanisms and impact on genomic stability.
Sign Up to like & getrecommendations! Published in 2019 at "DNA repair"
DOI: 10.1016/j.dnarep.2019.102654
Abstract: Replication of DNA is a fundamental biological process that ensures precise duplication of the genome and thus safeguards inheritance. Any errors occurring during this process must be repaired before the cell divides, by activating the… read more here.
Keywords: replication fork; dna; fork speed; speed ... See more keywords
Proteolytic control of genome integrity at the replication fork.
Sign Up to like & getrecommendations! Published in 2019 at "DNA repair"
DOI: 10.1016/j.dnarep.2019.102657
Abstract: Faithful duplication of the genome is critical for the survival of an organism and prevention of malignant transformation. Accurate replication of a large amount of genetic information in a timely manner is one of the… read more here.
Keywords: replication fork; dna replication; replication; genome integrity ... See more keywords
Quinacrine based gold hybrid nanoparticle caused apoptosis through modulating replication fork in oral cancer stem cells.
Sign Up to like & getrecommendations! Published in 2020 at "Molecular pharmaceutics"
DOI: 10.1021/acs.molpharmaceut.0c00197
Abstract: Presence of cancer stem cells (CSCs) in tumor micro environment is responsible for development of chemo-resistance and recurrence of cancer. Our previous investigation revealed the anti-cancer mechanism of quinacrine based silver and gold hybrid nanoparticle… read more here.
Keywords: replication fork; cancer stem; oral cancer; cancer ... See more keywords
Germline polymorphisms in genes maintaining the replication fork predict the efficacy of oxaliplatin and irinotecan in patients with metastatic colorectal cancer.
Sign Up to like & getrecommendations! Published in 2021 at "British journal of cancer"
DOI: 10.1038/s41416-021-01592-7
Abstract: BACKGROUND The TIMELESS-TIPIN complex protects the replication fork from replication stress induced by chemotherapeutic drugs. We hypothesised genetic polymorphisms of the TIMELESS-TIPIN complex may affect the response, progression-free survival (PFS), and overall survival (OS) of… read more here.
Keywords: replication fork; metastatic colorectal; bevacizumab; patients metastatic ... See more keywords
Mechanism for inverted-repeat recombination induced by a replication fork barrier
Sign Up to like & getrecommendations! Published in 2022 at "Nature Communications"
DOI: 10.1038/s41467-021-27443-w
Abstract: Replication stress and abundant repetitive sequences have emerged as primary conditions underlying genomic instability in eukaryotes. To gain insight into the mechanism of recombination between repeated sequences in the context of replication stress, we used… read more here.
Keywords: replication fork; recombination; barrier; replication ... See more keywords
Monitoring genome-wide replication fork directionality by Okazaki fragment sequencing in mammalian cells
Sign Up to like & getrecommendations! Published in 2021 at "Nature Protocols"
DOI: 10.1038/s41596-020-00454-5
Abstract: The ability to monitor DNA replication fork directionality at the genome-wide scale is paramount for a greater understanding of how genetic and environmental perturbations can impact replication dynamics in human cells. Here we describe a… read more here.
Keywords: replication fork; genome wide; fork directionality; mammalian cells ... See more keywords
Orc6 is a component of the replication fork and enables efficient mismatch repair
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2121406119
Abstract: Significance Origin recognition complex (ORC) is required for the initiation of DNA replication. Unlike other ORC components, the role of human Orc6 in replication remains to be resolved. We identified an unexpected role for hOrc6,… read more here.
Keywords: replication; replication fork; mismatch repair;
Replication fork regression and its regulation.
Sign Up to like & getrecommendations! Published in 2017 at "FEMS yeast research"
DOI: 10.1093/femsyr/fow110
Abstract: One major challenge during genome duplication is the stalling of DNA replication forks by various forms of template blockages. As these barriers can lead to incomplete replication, multiple mechanisms have to act concertedly to correct… read more here.
Keywords: replication fork; regression; fork regression; replication forks ... See more keywords
Functional analysis of germline RAD51C missense variants highlight the role of RAD51C in replication fork protection.
Sign Up to like & getrecommendations! Published in 2022 at "Human molecular genetics"
DOI: 10.1093/hmg/ddac281
Abstract: Monoallelic or biallelic RAD51C germline mutations results in chromosome instability disorders such as fanconi anemia (FA) and cancers. The bona fide function of RAD51C is to assist RAD51 nucleoprotein filament onto single strand DNA to… read more here.
Keywords: rad51c; replication; replication fork; fork protection ... See more keywords