Apoptosis detection via automated algorithms to analyze biomarker translocation in reporter cells
Sign Up to like & getrecommendations! Published in 2020 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.27280
Abstract: Rapid, efficient, and robust quantitative analyses of dynamic apoptotic events are essential in a high‐throughput screening workflow. Currently used methods have several bottlenecks, specifically, limitations in available fluorophores for downstream assays and misinterpretation of statistical… read more here.
Keywords: reporter; cell lines; reporter cells; apoptotic events ... See more keywords
A Conditionally Fluorescent Peptide Reporter of Secondary Structure Modulation
Sign Up to like & getrecommendations! Published in 2019 at "ChemBioChem"
DOI: 10.1002/cbic.201800377
Abstract: Proteins containing intrinsic disorder often form secondary structure upon interaction with a binding partner. Modulating such structures presents an approach for manipulating the resultant functional outcomes. Translational repressor protein 4E‐BP1 is an example of an… read more here.
Keywords: reporter; fluorescent peptide; conditionally fluorescent; secondary structure ... See more keywords
Heterogeneity and genomic loci of ubiquitous transgenic Cre reporter lines in zebrafish
Sign Up to like & getrecommendations! Published in 2022 at "Developmental Dynamics"
DOI: 10.1002/dvdy.499
Abstract: The most‐common strategy for zebrafish Cre/lox‐mediated lineage labeling experiments combines ubiquitously expressed, lox‐based Switch reporter transgenes with tissue‐specific Cre or 4‐OH‐Tamoxifen‐inducible CreERT2 driver lines. Although numerous Cre driver lines have been produced, only a few… read more here.
Keywords: reporter lines; cre; reporter; heterogeneity genomic ... See more keywords
A novel Cre‐inducible knock‐in ARL13B‐tRFP fusion cilium reporter
Sign Up to like & getrecommendations! Published in 2017 at "genesis"
DOI: 10.1002/dvg.23073
Abstract: Cilia play a major role in the regulation of numerous signaling pathways and are essential for embryonic development. Mutations in genes affecting ciliary function can cause a variety of diseases in humans summarized as ciliopathies.… read more here.
Keywords: reporter; cilium; arl13b trfp; trfp fusion ... See more keywords
Using a modified piggyBac transposon-combined Cre/loxP system to produce selectable reporter-free transgenic bovine mammary epithelial cells for somatic cell nuclear transfer.
Sign Up to like & getrecommendations! Published in 2023 at "Genesis"
DOI: 10.1002/dvg.23510
Abstract: Transposon systems are widely used for genetic engineering in various model organisms. PiggyBac (PB) has recently been confirmed to have highly efficient transposition in the mouse germ line and mammalian cell lines. In this study,… read more here.
Keywords: system; cell; reporter; bovine mammary ... See more keywords
Transient Cnp expression by early progenitors causes Cre‐Lox‐based reporter lines to map profoundly different fates
Sign Up to like & getrecommendations! Published in 2017 at "Glia"
DOI: 10.1002/glia.23095
Abstract: NG2 expressing oligodendroglial precursor cells are ubiquitous in the central nervous system and the only cell type cycling throughout life. Previous fate mapping studies have remained inconsistent regarding the question whether NG2 cells are capable… read more here.
Keywords: reporter; rosa26 yfp; cre lox; fate ... See more keywords
Establishment of a high‐sensitivity reporter system in mammalian cells for detecting juvenoids using juvenile hormone receptors of Daphnia pulex
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Applied Toxicology"
DOI: 10.1002/jat.3713
Abstract: Environmental waters are polluted by various chemicals originating from human activities. Recently, the environmental risk of juvenile hormones (JHs) to aquatic microcrustaceans has been recognized by risk assessors and researchers. JH is a major arthropod… read more here.
Keywords: reporter; receptors daphnia; risk; system ... See more keywords
Development of an HiBiT‐tagged reporter H3N2 influenza A virus and its utility as an antiviral screening platform
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Medical Virology"
DOI: 10.1002/jmv.28345
Abstract: The balance of the segmented genome derived from naturally occurring influenza A viruses (IAVs) is delicate and vulnerable to foreign insertions, thus most reporter IAVs up to date are generated using the backbone of the… read more here.
Keywords: platform; hibit tagged; reporter; influenza ... See more keywords
Generation of Tfap2c‐T2A‐tdTomato knock‐in reporter rats via adeno‐associated virus‐mediated efficient gene targeting
Sign Up to like & getrecommendations! Published in 2022 at "Molecular Reproduction and Development"
DOI: 10.1002/mrd.23562
Abstract: Gene editing in mammalian zygotes enables us to generate genetically modified animals rapidly and efficiently. In this study, we compare multiple gene targeting strategies in rat zygotes by generating a novel knock‐in reporter rat line… read more here.
Keywords: reporter; tfap2c t2a; gene targeting; tfap2c ... See more keywords
Isolation, Culture, and Motility Measurements of Epidermal Melanocytes from GFP-Expressing Reporter Mice.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/7651_2018_116
Abstract: In this article, we provide a method to isolate primary epidermal melanocytes from reporter mice, which also allow targeted gene inactivation. The mice from which these cells are isolated are bred into a Rosa26mT/mG reporter… read more here.
Keywords: reporter; reporter mice; isolation culture; epidermal melanocytes ... See more keywords
NPY-mRFP Rat Basophilic Leukemia (RBL) Reporter: A Novel, Fast Reporter of Basophil/Mast Cell Degranulation.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0696-4_13
Abstract: Humanized rat basophilic leukemia (RBL) reporter cell lines are increasingly used for the detection of allergen-specific IgE and other purposes, such as the detection of allergens and standardization of allergen preparations. Existing reporter systems have… read more here.
Keywords: reporter; rat basophilic; basophilic leukemia; npy mrfp ... See more keywords