High-Content Reporter Assays.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7724-6_13
Abstract: While luminescent reporter gene assays allow for a rapid and relatively interference free assessment of the activation state of a luminescent reporter, fluorescent reporters do not. They suffer from artifacts such as compound fluorescence and… read more here.
Keywords: reporter; reporter assays; biology; content reporter ... See more keywords
Linear models enable powerful differential activity analysis in massively parallel reporter assays
Sign Up to like & getrecommendations! Published in 2019 at "BMC Genomics"
DOI: 10.1186/s12864-019-5556-x
Abstract: BackgroundMassively parallel reporter assays (MPRAs) have emerged as a popular means for understanding noncoding variation in a variety of conditions. While a large number of experiments have been described in the literature, analysis typically uses… read more here.
Keywords: analysis; reporter assays; parallel reporter; models enable ... See more keywords
MPRAnalyze: statistical framework for massively parallel reporter assays
Sign Up to like & getrecommendations! Published in 2019 at "Genome Biology"
DOI: 10.1186/s13059-019-1787-z
Abstract: Massively parallel reporter assays (MPRAs) can measure the regulatory function of thousands of DNA sequences in a single experiment. Despite growing popularity, MPRA studies are limited by a lack of a unified framework for analyzing… read more here.
Keywords: framework; massively parallel; reporter assays; parallel reporter ... See more keywords
Characterization of De Novo Promoter Variants in Autism Spectrum Disorder with Massively Parallel Reporter Assays
Sign Up to like & getrecommendations! Published in 2023 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms24043509
Abstract: Autism spectrum disorder (ASD) is a common, complex, and highly heritable condition with contributions from both common and rare genetic variations. While disruptive, rare variants in protein-coding regions clearly contribute to symptoms, the role of… read more here.
Keywords: autism spectrum; parallel reporter; spectrum disorder; reporter assays ... See more keywords