Apoptosis detection via automated algorithms to analyze biomarker translocation in reporter cells
Sign Up to like & getrecommendations! Published in 2020 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.27280
Abstract: Rapid, efficient, and robust quantitative analyses of dynamic apoptotic events are essential in a high‐throughput screening workflow. Currently used methods have several bottlenecks, specifically, limitations in available fluorophores for downstream assays and misinterpretation of statistical… read more here.
Keywords: reporter; cell lines; reporter cells; apoptotic events ... See more keywords
Reporter cell assay-based functional quantification of TNF-α-antagonists in serum -- a proof-of-principle study for adalimumab.
Sign Up to like & getrecommendations! Published in 2020 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2020.113646
Abstract: Quantification of therapeutic antibodies is commonly based on physico-chemical assays such as enzyme-linked immunoabsorption assays (ELISA) and lately on mass spectrometry. However, the functional integrity of evaluated immunoglobulins is yet not assessed. Consequently, a commercially… read more here.
Keywords: cell assay; quantification; reporter cell; functional quantification ... See more keywords
Reporter cell lines for detection of pertussis toxin in acellular pertussis vaccines as a functional animal-free alternative to the in vivo histamine sensitization test.
Sign Up to like & getrecommendations! Published in 2017 at "Vaccine"
DOI: 10.1016/j.vaccine.2017.01.011
Abstract: Detoxified pertussis toxin (pertussis toxoid) is a major antigen in acellular pertussis vaccines. Testing these vaccines on the presence of residual pertussis toxin (PTx) and reversion to toxicity is performed by the regulatory required in… read more here.
Keywords: cell lines; reporter cell; pertussis; cell ... See more keywords
Effects of Flavored Nonalcoholic Beverages on Transcriptional Activities of Nuclear and Steroid Hormone Receptors: Proof of Concept for Novel Reporter Cell Line PAZ-PPARg.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.8b05158
Abstract: We developed and characterized a novel human luciferase reporter cell line for the assessment of peroxisome proliferator-activated receptor γ (PPARγ) transcriptional activity, PAZ-PPARg. The luciferase activity induced by PPARγ endogenous agonist 15d-PGJ2 and prostaglandin PGD2… read more here.
Keywords: transcriptional activities; cell line; paz pparg; reporter cell ... See more keywords
Tales of 1,008 small molecules: phenomic profiling through live-cell imaging in a panel of reporter cell lines
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-69354-8
Abstract: Phenomic profiles are high-dimensional sets of readouts that can comprehensively capture the biological impact of chemical and genetic perturbations in cellular assay systems. Phenomic profiling of compound libraries can be used for compound target identification… read more here.
Keywords: live cell; phenomic profiling; cell lines; reporter cell ... See more keywords
High variability in toxicity of welding fume nanoparticles from stainless steel in lung cells and reporter cell lines: the role of particle reactivity and solubility
Sign Up to like & getrecommendations! Published in 2019 at "Nanotoxicology"
DOI: 10.1080/17435390.2019.1650972
Abstract: Abstract Millions of people in the world perform welding as their primary occupation resulting in exposure to metal-containing nanoparticles in the fumes generated. Even though health effects including airway diseases are well-known, there is currently… read more here.
Keywords: fume particles; toxicity; welding fume; metal ... See more keywords
Validation of a Reporter Cell Line for Flavivirus Inhibition Assays
Sign Up to like & getrecommendations! Published in 2023 at "Microbiology Spectrum"
DOI: 10.1128/spectrum.05027-22
Abstract: The Hec1a-IFNB-Luc reporter cell line allows the detection of all 10 flaviviruses tested, including the 4 dengue virus serotypes. Its use for serodiagnostic purposes, measuring neutralizing antibody activity in sera, and the assessment of the… read more here.
Keywords: cell line; hec1a ifnb; reporter cell;
Development and Characterisation of a Novel NF-κB Reporter Cell Line for Investigation of Neuroinflammation
Sign Up to like & getrecommendations! Published in 2017 at "Mediators of Inflammation"
DOI: 10.1155/2017/6209865
Abstract: Aberrant activation of the transcription factor NF-κB, as well as uncontrolled inflammation, has been linked to autoimmune diseases, development and progression of cancer, and neurological disorders like Alzheimer's disease. Reporter cell lines are a valuable… read more here.
Keywords: reporter; line investigation; cell line; reporter cell ... See more keywords
A reporter cell line for the automated quantification of SARS-CoV-2 infection in living cells
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2022.1031204
Abstract: The SARS-CoV-2 pandemic and the urgent need for massive antiviral testing highlighted the lack of a good cell-based assay that allowed for a fast, automated screening of antivirals in high-throughput content with minimal handling requirements… read more here.
Keywords: cell; cell line; living cells; reporter cell ... See more keywords