Use of Bacterial Luciferase as a Reporter Gene in Eukaryotic Systems.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1258-3_6
Abstract: Reporter gene assays are powerful tools for monitoring dynamic molecular changes and for evaluating the responses that occur at the genetic elements within cells in response to exogenous molecules. In general, various protein systems can… read more here.
Keywords: reporter; reporter gene; bacterial luciferase; use bacterial ... See more keywords
Making It All Work: Functional Genomics and Reporter Gene Assays.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7724-6_7
Abstract: Functional genomics is the study of the function of genes on a genome-wide level. Reporter gene assays can be utilized in this context to dissect signaling cascades, find new drug targets, or decipher the function… read more here.
Keywords: genome wide; reporter gene; functional genomics; biology ... See more keywords
Reporter Gene Assays Using Transfectable Functional Genomics Libraries.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7724-6_8
Abstract: Transfectable functional genomics libraries are traditionally the workhorses of functional genomics screening using reporter gene assays. These libraries offer insight into fundamental cellular processes governing health and disease and can be utilized in an arrayed… read more here.
Keywords: reporter gene; genomics libraries; functional genomics; transfectable functional ... See more keywords
High-Throughput Screening of Chemical Compound Libraries for Modulators of Salicylic Acid Signaling by In Situ Monitoring of Glucuronidase-Based Reporter Gene Expression.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7874-8_5
Abstract: Salicylic acid (SA) is a vital phytohormone that is intimately involved in coordination of the complex plant defense response to pathogen attack. Many aspects of SA signaling have been unraveled by classical genetic and biochemical… read more here.
Keywords: reporter; methodology; salicylic acid; reporter gene ... See more keywords
Comparison of lacZ reporter gene expression in gilthead sea bream (Sparus aurata) following oral or intramuscular administration of plasmid DNA in chitosan nanoparticles
Sign Up to like & getrecommendations! Published in 2017 at "Aquaculture"
DOI: 10.1016/j.aquaculture.2017.03.023
Abstract: Abstract The development of non-parenteral delivery systems allowing efficient intestinal absorption in fish is a current research topic drawing much interest. Different aspects would benefit from such oral delivery, especially those related to immunization, owing… read more here.
Keywords: administration; reporter gene; chitosan nanoparticles; gene expression ... See more keywords
Induction of AhR transactivation by PBDD/Fs and PCDD/Fs using a novel human-relevant, high-throughput DRhuman CALUX reporter gene assay.
Sign Up to like & getrecommendations! Published in 2021 at "Chemosphere"
DOI: 10.1016/j.chemosphere.2020.128086
Abstract: Polychlorinated dioxins and dibenzofurans (PCDD/Fs) are highly toxic contaminants that are strictly regulated and monitored in the environment and food to reduce human exposure. Recently, the increasing occurrence of polybrominated dioxins and dibenzofurans (PBDD/Fs) in… read more here.
Keywords: human relevant; transactivation; reporter gene; ahr ... See more keywords
Reporter gene imaging and its role in imaging-based drug development.
Sign Up to like & getrecommendations! Published in 2020 at "Drug discovery today"
DOI: 10.1016/j.drudis.2019.12.010
Abstract: Reporter gene imaging (RGI) is described as the methodology that involves imaging of the encoding proteins that can be used as surrogate markers when fused with regulatory regions of the gene of interest. It provides… read more here.
Keywords: reporter gene; imaging role; drug; role imaging ... See more keywords
Characterization of a reliable cell-based reporter gene assay for measuring bioactivities of therapeutic anti-interleukin-23 monoclonal antibodies.
Sign Up to like & getrecommendations! Published in 2020 at "International immunopharmacology"
DOI: 10.1016/j.intimp.2020.106647
Abstract: Environmental disturbances may result in dysregulation of interleukin-23 (IL-23), which is a crucial modulator of immunity. Several therapeutic monoclonal antibodies (mAbs) have been developed for treating IL-23-related autoimmune inflammation, such as ustekinumab, guselkumab, tildrakizumab, and… read more here.
Keywords: gene assay; reporter gene; monoclonal antibodies; cell based ... See more keywords
Development of a robust bioassay of monoclonal antibodies and biosimilars against TNF-α by NF-κB-inducible lentiviral reporter gene.
Sign Up to like & getrecommendations! Published in 2021 at "International immunopharmacology"
DOI: 10.1016/j.intimp.2021.107418
Abstract: The tumor necrosis factor alpha (TNF-α)/nuclear factor-kappa B (NF-κB) signaling pathway plays a crucial role in the pathogenesis of inflammatory diseases. Several therapeutic monoclonal antibodies (mAbs) and biosimilars against TNF-α have been developed to treat… read more here.
Keywords: monoclonal antibodies; reporter gene; biosimilars tnf; inducible lentiviral ... See more keywords
Development and validation of a novel reporter gene assay for determination of recombinant human thrombopoietin.
Sign Up to like & getrecommendations! Published in 2021 at "International immunopharmacology"
DOI: 10.1016/j.intimp.2021.107982
Abstract: Recombinant human thrombopoietin (rhTPO) was approved by the National Medical Products Administration in 2010 for the treatment of thrombocytopenia in patients with immune thrombocytopenic purpura and chemotherapy-induced thrombocytopenia. Nevertheless, no method for determining rhTPO bioactivity… read more here.
Keywords: reporter; recombinant human; gene assay; reporter gene ... See more keywords
Development of a robust reporter gene based assay for the bioactivity determination of IL‐5‐targeted therapeutic antibodies
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Pharmaceutical and Biomedical Analysis"
DOI: 10.1016/j.jpba.2017.09.032
Abstract: Graphical abstract Figure. No Caption available. HighlightsA reporter gene assay for bioactivity determination of the anti‐IL‐5 mAb was established, validated and the procedure was optimized.The novel assay was not only highly consistent with the anti‐proliferation… read more here.
Keywords: reporter; bioactivity; reporter gene; bioactivity determination ... See more keywords