Molecular Genetic Characterization of Mutagenesis Using a Highly Sensitive Single-Stranded DNA Reporter System in Budding Yeast.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7306-4_4
Abstract: Mutations are permanent alterations to the coding content of DNA. They are starting material for the Darwinian evolution of species by natural selection, which has yielded an amazing diversity of life on Earth. Mutations can… read more here.
Keywords: dna; reporter system; molecular genetic; highly sensitive ... See more keywords
lacZ Reporter System as a Tool to Study Virulence Gene Regulation in Bacterial Pathogens.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7604-1_5
Abstract: β-galactosidase assay has been established as one of the most widely used reporters and can be effectually exploited to study promoter activity of Salmonella and other pathogens under various conditions. This method includes a preliminary… read more here.
Keywords: system; reporter system; study; promoter ... See more keywords
Effective removal of a range of Ti/Ri plasmids using a pBBR1-type vector having a repABC operon and a lux reporter system
Sign Up to like & getrecommendations! Published in 2017 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-017-8721-7
Abstract: Ti and Ri plasmids of pathogenic Agrobacterium strains are stably maintained by the function of a repABC operon and have been classified into four incompatibility groups, namely, incRh1, incRh2, incRh3, and incRh4. Removal of these… read more here.
Keywords: vector; removal; reporter system; repabc operon ... See more keywords
Visualization of tandem repeat mutagenesis in Bacillus subtilis.
Sign Up to like & getrecommendations! Published in 2018 at "DNA repair"
DOI: 10.1016/j.dnarep.2018.01.006
Abstract: Mutations are crucial for the emergence and evolution of proteins with novel functions, and thus for the diversity of life. Tandem repeats (TRs) are mutational hot spots that are present in the genomes of all… read more here.
Keywords: mutagenesis; reporter system; mutation reporter; mutation ... See more keywords
An effector–reporter system to study cellular signal transduction in strawberry fruit (Fragaria ananassa)
Sign Up to like & getrecommendations! Published in 2021 at "Horticulture Research"
DOI: 10.1038/s41438-021-00493-3
Abstract: An effector–reporter system is a powerful tool used to study cellular signal transduction, but this technique has been traditionally used in protoplasts. A similar system to study cellular signal transduction in fruits has not yet… read more here.
Keywords: reporter; study; reporter system; effector reporter ... See more keywords
A dual-reporter system for investigating and optimizing protein translation and folding in E. coli.
Sign Up to like & getrecommendations! Published in 2021 at "Nature communications"
DOI: 10.1038/s41467-021-26337-1
Abstract: Strategies for investigating and optimizing the expression and folding of proteins for biotechnological and pharmaceutical purposes are in high demand. Here, we describe a dual-reporter biosensor system that simultaneously assesses in vivo protein translation and… read more here.
Keywords: reporter; system; reporter system; protein translation ... See more keywords
A reporter system for enriching CRISPR/Cas9 knockout cells in technically challenging settings like patient models
Sign Up to like & getrecommendations! Published in 2021 at "Scientific Reports"
DOI: 10.1038/s41598-021-91760-9
Abstract: CRISPR/Cas9 represents a valuable tool to determine protein function, but technical hurdles limit its use in challenging settings such as cells unable to grow in vitro like primary leukemia cells and xenografts derived thereof (PDX).… read more here.
Keywords: reporter; crispr cas9; challenging settings; reporter system ... See more keywords
Establishment of an in vitro reporter system for screening HBx-targeting molecules.
Sign Up to like & getrecommendations! Published in 2019 at "Acta biochimica et biophysica Sinica"
DOI: 10.1093/abbs/gmz016
Abstract: Chronic hepatitis B virus (HBV) infection remains a global public health problem. HBV-encoded X protein (HBx) is a multifunctional regulator that is required to initiate and maintain productive HBV infection, and is involved in HBV-related… read more here.
Keywords: system screening; system; hbx targeting; reporter system ... See more keywords
Species-Specific Enhancer Activity of OCT4 in Porcine Pluripotency: The Porcine OCT4 Reporter System Could Monitor Pluripotency in Porcine Embryo Development and Embryonic Stem Cells
Sign Up to like & getrecommendations! Published in 2022 at "Stem Cells International"
DOI: 10.1155/2022/6337532
Abstract: The present study examined the activity and function of the pig OCT4 enhancer in the porcine early embryonic development stage and porcine authentic embryonic stem cells. OCT4 is known as a pluripotent regulator, and its… read more here.
Keywords: development; pluripotency; reporter system; pluripotency porcine ... See more keywords
LuxCDE-luxAB-based promoter reporter system to monitor the Yersinia enterocolitica O:3 gene expression in vivo
Sign Up to like & getrecommendations! Published in 2017 at "PLoS ONE"
DOI: 10.1371/journal.pone.0172877
Abstract: It is crucial to understand the in vitro and in vivo regulation of the virulence factor genes of bacterial pathogens. In this study, we describe the construction of a versatile reporter system for Yersinia enterocolitica… read more here.
Keywords: reporter; monitor; reporter system; promoter ... See more keywords
Antigen-Dependent Inducible T-Cell Reporter System for PET Imaging of Breast Cancer and Glioblastoma
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Nuclear Medicine"
DOI: 10.2967/jnumed.122.264284
Abstract: Visual Abstract For the past several decades, chimeric antigen receptor T-cell therapies have shown promise in the treatment of cancers. These treatments would greatly benefit from companion imaging biomarkers to follow the trafficking of T… read more here.
Keywords: cell; reporter; pet; reporter system ... See more keywords