Extended-representation bisulfite sequencing of gene regulatory elements in multiplexed samples and single cells.
Sign Up to like & getrecommendations! Published in 2021 at "Nature biotechnology"
DOI: 10.1038/s41587-021-00910-x
Abstract: The biological roles of DNA methylation have been elucidated by profiling methods based on whole-genome or reduced-representation bisulfite sequencing, but these approaches do not efficiently survey the vast numbers of non-coding regulatory elements in mammalian… read more here.
Keywords: representation bisulfite; bisulfite sequencing; regulatory elements; extended representation ... See more keywords
epiGBS2: Improvements and evaluation of highly multiplexed, epiGBS-based reduced representation bisulfite sequencing.
Sign Up to like & getrecommendations! Published in 2022 at "Molecular ecology resources"
DOI: 10.1111/1755-0998.13597
Abstract: Several reduced representation bisulfite sequencing methods have been developed in recent years to determine cytosine methylation de novo in non-model species. Here, we present epiGBS2, a laboratory protocol based on epiGBS (Gurp et al., 2016)… read more here.
Keywords: pipeline; reduced representation; representation bisulfite; bisulfite sequencing ... See more keywords