Residue-Specific Incorporation of Noncanonical Amino Acids for Protein Engineering.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7574-7_8
Abstract: The incorporation of noncanonical amino acids has given protein chemists access to an expanded repertoire of amino acids. This methodology has significantly broadened the scope of protein engineering allowing introduction of amino acids with non-native… read more here.
Keywords: noncanonical amino; amino; amino acids; protein engineering ... See more keywords
Residue-specific binding mechanisms of Thioflavin T to a surface of flat β-sheets within a peptide self-assembly mimic.
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DOI: 10.1021/acs.biochem.0c00280
Abstract: Thioflavin T (ThT) is a popular fluorescent dye for detecting amyloid, a protein aggregate with a β-sheet-rich structure that causes many neurodegenerative diseases. Despite the dye's popularity, a detailed understanding of its molecular binding mechanism… read more here.
Keywords: specific binding; binding mechanisms; binding mechanism; tht ... See more keywords
In vivo biosynthesis of tyrosine analogs and their concurrent incorporation into a residue-specific manner for enzyme engineering.
Sign Up to like & getrecommendations! Published in 2019 at "Chemical communications"
DOI: 10.1039/c9cc08503c
Abstract: Herein we report the development of an efficient cellular system for the in vivo biosynthesis of Tyr-analogs and their concurrent incorporation into target proteins by the residue-specific approach. This system makes use of common phenol… read more here.
Keywords: vivo biosynthesis; analogs concurrent; concurrent incorporation; residue specific ... See more keywords