High-resolution melt curve PCR assay for specific detection of E. coli O157:H7 in beef
Sign Up to like & getrecommendations! Published in 2018 at "Food Control"
DOI: 10.1016/j.foodcont.2017.11.025
Abstract: Abstract Among the disease-causing Shiga toxin-producing Escherichia coli (STEC), E. coli O157:H7 is estimated to cause one-third of the total STEC illnesses and the most cases of hemolytic uremic syndrome (HUS) in the U.S. The uidA… read more here.
Keywords: beef; high resolution; coli o157; pcr assay ... See more keywords
Speciation of common Gram-negative pathogens using a highly multiplexed high resolution melt curve assay
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-18915-5
Abstract: The identification of the bacterial species responsible for an infection remains an important step for the selection of antimicrobial therapy. Gram-negative bacteria are an important source of hospital and community acquired infections and frequently antimicrobial… read more here.
Keywords: high resolution; common gram; gram negative; gram ... See more keywords
Differentiation of palm-infecting phytoplasmas in the Caribbean basin using high resolution melt curve analysis of the secA gene.
Sign Up to like & getrecommendations! Published in 2022 at "Plant disease"
DOI: 10.1094/pdis-02-22-0393-re
Abstract: Palm lethal decline is a disease is always fatal to infected palm hosts and is caused by three species of phytoplasma in the Caribbean basin; 'Candidatus Phytoplasma palmae', 'Ca. P. aculeata', and 'Ca. P. hispanola'.… read more here.
Keywords: caribbean basin; melt curve; curve analysis; high resolution ... See more keywords
High‐resolution melt analysis without DNA extraction affords rapid genotype resolution and species identification
Sign Up to like & getrecommendations! Published in 2017 at "Molecular Ecology Resources"
DOI: 10.1111/1755-0998.12599
Abstract: Extracting and sequencing DNA from specimens can impose major time and monetary costs to studies requiring genotyping, or identification to species, of large numbers of individuals. As such, so‐called direct PCR methods have been developed… read more here.
Keywords: resolution; high resolution; pcr; resolution melt ... See more keywords