Dispersion from Cα or NH: 4D experiments for backbone resonance assignment of intrinsically disordered proteins
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Biomolecular Nmr"
DOI: 10.1007/s10858-020-00299-w
Abstract: Resonance assignment of intrinsically disordered proteins is remarkably challenging due to scant chemical shift dispersion arising from conformational heterogeneity. The challenge is even greater if repeating segments are present in the amino acid sequence. To… read more here.
Keywords: resonance assignment; assignment intrinsically; disordered proteins; intrinsically disordered ... See more keywords
Backbone resonance assignment for the N-terminal region of bacterial tRNA-(N1G37) methyltransferase
Sign Up to like & getrecommendations! Published in 2019 at "Biomolecular NMR Assignments"
DOI: 10.1007/s12104-018-9849-9
Abstract: Bacterial tRNA (guanine37-N1)-methyltransferase (TrmD) is an important antibacterial target due to its essential role in translation. TrmD has two domains connected with a flexible linker. The N-terminal domain (NTD) of TrmD contains the S-adenosyl-l-methionine (SAM)… read more here.
Keywords: backbone resonance; methyltransferase; trna; resonance assignment ... See more keywords
Resonance assignment of the 128 kDa enzyme I dimer from Thermoanaerobacter tengcongensis
Sign Up to like & getrecommendations! Published in 2019 at "Biomolecular NMR Assignments"
DOI: 10.1007/s12104-019-09893-y
Abstract: Enzyme I (EI) of the bacterial phosphotransferase system (PTS) utilizes phosphoenolpyruvate (PEP) as a source of energy in order to transport sugars across the cellular membrane. PEP binding to EI initiates a phosphorylation cascade that… read more here.
Keywords: assignment 128; resonance assignment; thermoanaerobacter tengcongensis; 128 kda ... See more keywords
Backbone resonance assignment of PDI b'xa' domain construct.
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DOI: 10.1007/s12104-021-10038-3
Abstract: Human protein disulfide isomerase (PDI), a protein containing 4 domains a, b, b', a', disordered x linker and C-terminus, plays critical roles in disulfide bond reactions and proper protein folding in the endoplasmic reticulum. The… read more here.
Keywords: backbone resonance; construct; domain; assignment pdi ... See more keywords
Resonance assignment and secondary structure of DbpA protein from the European species, Borrelia afzelii
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DOI: 10.1007/s12104-021-10039-2
Abstract: Decorin binding proteins (Dbps) mediate attachment of spirochetes in host organisms during the early stages of Lyme disease infection. Previously, different binding mechanisms of Dbps to glycosaminoglycans have been elucidated for the pathogenic species Borrelia… read more here.
Keywords: species borrelia; assignment secondary; secondary structure; structure ... See more keywords
Co-refolding of a functional complex of Dengue NS3 protease and NS2B co-factor domain and backbone resonance assignment by solution NMR.
Sign Up to like & getrecommendations! Published in 2017 at "Protein expression and purification"
DOI: 10.1016/j.pep.2017.07.002
Abstract: A novel approach for separate expression of dengue virus NS3 protease and its NS2B cofactor domain is described in this paper. The two proteins are expressed in E.coli and purified separately and subsequently efficiently co-refolded… read more here.
Keywords: backbone resonance; protease ns2b; ns3 protease; resonance ... See more keywords
Combining asymmetric 13C-labeling and isotopic filter/edit NOESY: a novel strategy for rapid and logical RNA resonance assignment
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DOI: 10.1093/nar/gkx591
Abstract: Abstract Although ∼98% of the human genomic output is transcribed as non-protein coding RNA, read more here.
Keywords: novel strategy; resonance assignment; rna; assignment ... See more keywords
Strategies for RNA Resonance Assignment by 13C/15N- and 1H-Detected Solid-State NMR Spectroscopy
Sign Up to like & getrecommendations! Published in 2021 at "Frontiers in Molecular Biosciences"
DOI: 10.3389/fmolb.2021.743181
Abstract: Magic angle spinning (MAS) solid-state NMR (ssNMR) is an established tool that can be applied to non-soluble or non-crystalline biomolecules of any size or complexity. The ssNMR method advances rapidly due to technical improvements and… read more here.
Keywords: solid state; state nmr; 13c 15n; rna resonance ... See more keywords