Toward Direct Observation of the DNA Binding Dynamics of Monomeric Type IIP Restriction Endonucleases
Sign Up to like & getrecommendations! Published in 2017 at "Biophysical Journal"
DOI: 10.1016/j.bpj.2016.11.939
Abstract: Restriction endonucleases (REases) are enzymes that cleave duplex DNA at a recognition site specified by a particular nucleotide sequence. These enzymes are found in a wide range of bacterial species, and serve as a defense… read more here.
Keywords: restriction endonucleases; dna binding; type iip; dna ... See more keywords
Restriction endonucleases that cleave RNA/DNA heteroduplexes bind dsDNA in A-like conformation
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DOI: 10.1093/nar/gkaa403
Abstract: Abstract Restriction endonucleases naturally target DNA duplexes. Systematic screening has identified a small minority of these enzymes that can also cleave RNA/DNA heteroduplexes and that may therefore be useful as tools for RNA biochemistry. We… read more here.
Keywords: restriction endonucleases; cleave rna; dna; dna heteroduplexes ... See more keywords
Golden Gate assembly of BioBrick-compliant parts using Type II restriction endonucleases.
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DOI: 10.2144/btn-2021-0083
Abstract: Aims: New methods of DNA recombination that capture the principal advantages of the BioBrick standard (ease of design) and Golden Gate assembly (decreased labor) are demonstrated here. Methods & materials: Both methods employ DNA methyltransferase… read more here.
Keywords: golden gate; restriction endonucleases; gate assembly; assembly ... See more keywords