Terminal Repeat Analysis of EBV Genomes.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6655-4_12
Abstract: Epstein-Barr virus (EBV) was the first human virus associated directly with human malignancies. During EBV infection of various host cells the double-stranded linear EBV DNA carried by the virions undergoes circularization. Since there are variable… read more here.
Keywords: ebv; terminal restriction; analysis; ebv genomes ... See more keywords
Generation of 3C Libraries from Cross-Linked Cells.
Sign Up to like & getrecommendations! Published in 2018 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot097840
Abstract: This protocol, which describes how 3C libraries are made from formaldehyde-fixed mammalian cells, starts with cells that were previously cross-linked. Before embarking on a 3C-based analysis, decide which restriction enzyme will be used. Two parameters… read more here.
Keywords: restriction; use restriction; cross linked; generation libraries ... See more keywords
DNA methylation estimation using methylation-sensitive restriction enzyme bisulfite sequencing (MREBS)
Sign Up to like & getrecommendations! Published in 2019 at "PLoS ONE"
DOI: 10.1371/journal.pone.0214368
Abstract: Whole-genome bisulfite sequencing (WGBS) and reduced representation bisulfite sequencing (RRBS) are widely used for measuring DNA methylation levels on a genome-wide scale. Both methods have limitations: WGBS is expensive and prohibitive for most large-scale projects;… read more here.
Keywords: methylation; bisulfite sequencing; sensitive restriction; dna methylation ... See more keywords