New bifunctional restriction-modification enzyme AloI isoschizomer (PcoI): Bioinformatics analysis, purification and activity confirmation.
Sign Up to like & getrecommendations! Published in 2018 at "Gene"
DOI: 10.1016/j.gene.2018.03.069
Abstract: Type II restriction endonucleases and modification DNA-methyltransferases are key instruments of genetic engineering. Recently the number of proteins assigned to this group exceeds 8500. Subtype IIC organizes bifunctional endonuclease-methyltransferase enzymes and currently consists of 16… read more here.
Keywords: restriction; restriction modification; modification; aloi isoschizomer ... See more keywords
An alternative approach to study the enzymatic specificities of the CfrBI restriction–modification system
Sign Up to like & getrecommendations! Published in 2019 at "Heliyon"
DOI: 10.1016/j.heliyon.2019.e01846
Abstract: Restriction–modification systems (RMS) are the main gene-engineering tools and a suitable model to study the molecular mechanisms of catalysis and DNA–protein interactions. Research into the catalytic properties of these enzymes, determination of hydrolysis and DNA-methylation… read more here.
Keywords: restriction; cfrbi restriction; site; restriction modification ... See more keywords
Coordination of phage genome degradation versus host genome protection by a bifunctional restriction-modification enzyme visualized by CryoEM.
Sign Up to like & getrecommendations! Published in 2021 at "Structure"
DOI: 10.1016/j.str.2021.03.012
Abstract: Restriction enzymes that combine methylation and cleavage into a single assemblage and modify one DNA strand are capable of efficient adaptation toward novel targets. However, they must reliably cleave invasive DNA and methylate newly replicated… read more here.
Keywords: modification enzyme; restriction; dna; restriction modification ... See more keywords
DNA methylation by three Type I restriction modification systems of Escherichia coli does not influence gene regulation of the host bacterium
Sign Up to like & getrecommendations! Published in 2021 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkab530
Abstract: Abstract DNA methylation is a common epigenetic mark that influences transcriptional regulation, and therefore cellular phenotype, across all domains of life. In particular, both orphan methyltransferases and those from phasevariable restriction modification systems (RMSs) have… read more here.
Keywords: methylation; restriction modification; modification systems; type ... See more keywords
A mobile restriction–modification system provides phage defence and resolves an epigenetic conflict with an antagonistic endonuclease
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac147
Abstract: Abstract Epigenetic DNA methylation plays an important role in bacteria by influencing gene expression and allowing discrimination between self-DNA and intruders such as phages and plasmids. Restriction–modification (RM) systems use a methyltransferase (MTase) to modify… read more here.
Keywords: system; dna; epigenetic conflict; system provides ... See more keywords
Molecular basis for lethal cross-talk between two unrelated bacterial transcription factors - the regulatory protein of a restriction-modification system and the repressor of a defective prophage
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac914
Abstract: Abstract Bacterial gene expression depends on the efficient functioning of global transcriptional networks, however their interconnectivity and orchestration rely mainly on the action of individual DNA binding proteins called transcription factors (TFs). TFs interact not… read more here.
Keywords: two unrelated; regulatory protein; repressor defective; transcription factors ... See more keywords
REBASE: a database for DNA restriction and modification: enzymes, genes and genomes
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac975
Abstract: Abstract REBASE is a comprehensive and extensively curated database of information about the components of restriction-modification (RM) systems. It is fully referenced and provides information about the recognition and cleavage sites for both restriction enzymes… read more here.
Keywords: database dna; rebase database; restriction modification; restriction ... See more keywords
The third restriction–modification system from Thermus aquaticus YT-1: solving the riddle of two TaqII specificities
Sign Up to like & getrecommendations! Published in 2017 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkx599
Abstract: Abstract Two restriction–modification systems have been previously discovered in Thermus aquaticus YT-1. TaqI is a 263-amino acid (aa) Type IIP restriction enzyme that recognizes and cleaves within the symmetric sequence 5′-TCGA-3′. TaqII, in contrast, is… read more here.
Keywords: restriction; taqii; restriction modification; thermus aquaticus ... See more keywords
A model for the evolution of prokaryotic DNA restriction-modification systems based upon the structural malleability of Type I restriction-modification enzymes
Sign Up to like & getrecommendations! Published in 2018 at "Nucleic Acids Research"
DOI: 10.1093/nar/gky760
Abstract: Abstract Restriction Modification (RM) systems prevent the invasion of foreign genetic material into bacterial cells by restriction and protect the host's genetic material by methylation. They are therefore important in maintaining the integrity of the… read more here.
Keywords: restriction; restriction modification; modification systems; type ... See more keywords
Transcriptome analyses of cells carrying the Type II Csp231I restriction–modification system reveal cross-talk between two unrelated transcription factors: C protein and the Rac prophage repressor
Sign Up to like & getrecommendations! Published in 2019 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkz665
Abstract: Abstract Restriction-modification (R–M) systems represent an effective mechanism of defence against invading bacteriophages, and are widely spread among bacteria and archaea. In acquiring a Type II R–M system via horizontal gene transfer, the new hosts… read more here.
Keywords: cross talk; restriction; transcription; system ... See more keywords
DNA Methylation by Restriction Modification Systems Affects the Global Transcriptome Profile in Borrelia burgdorferi
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Bacteriology"
DOI: 10.1128/jb.00395-18
Abstract: Lyme disease is the most prevalent vector-borne disease in North America and is classified by the Centers for Disease Control and Prevention (CDC) as an emerging infectious disease with an expanding geographical area of occurrence.… read more here.
Keywords: methylation; restriction modification; modification systems; gene ... See more keywords