Optimization of Enzyme Mechanism along the Evolutionary Trajectory of a Computationally Designed (Retro-)Aldolase.
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DOI: 10.1021/jacs.7b05796
Abstract: De novo biocatalysts have been successfully generated by computational design and subsequent experimental optimization. Here, we examined the evolutionary history of the computationally designed (retro-)aldolase RA95. The modest activity of the starting enzyme was previously… read more here.
Keywords: mechanism; designed retro; retro aldolase; computationally designed ... See more keywords