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   Articles with "reverse genetic" as a keyword



Development of the coronavirus reverse genetic system: Core technology for pathogenesis mechanisms research and vaccine/drug development

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recommendations! Published in 2025 at "Virulence"

DOI: 10.1080/21505594.2025.2525930

Abstract: ABSTRACT Coronaviruses (CoVs) are enveloped, single-stranded, positive-sense RNA viruses that cause respiratory, gastrointestinal, hepatic, and neurological diseases in humans and other animals. In recent years, frequent outbreaks of emerging and re-emerging CoVs have threatened animal… read more here.

Keywords: technology; drug; vaccine; reverse genetic ... See more keywords

A rescued NADC30-like virus by reverse genetic manipulation exhibits moderate virulence and a promising application perspective.

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recommendations! Published in 2022 at "Virus research"

DOI: 10.2139/ssrn.4046625

Abstract: NADC30-like porcine reproductive and respiratory syndrome virus (PRRSV), which is highly homologous to the NADC30 strain isolated in the United States. The NADC30-like PRRSV was first reported in 2014 in China, where it spread and… read more here.

Keywords: genetic manipulation; virulence; nadc30 like; reverse genetic ... See more keywords

Distribution and Functions of Monodehydroascorbate Reductases in Plants: Comprehensive Reverse Genetic Analysis of Arabidopsis thaliana Enzymes

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recommendations! Published in 2021 at "Antioxidants"

DOI: 10.3390/antiox10111726

Abstract: Monodehydroascorbate reductase (MDAR) is an enzyme involved in ascorbate recycling. Arabidopsis thaliana has five MDAR genes that encode two cytosolic, one cytosolic/peroxisomal, one peroxisomal membrane-attached, and one chloroplastic/mitochondrial isoform. In contrast, tomato plants possess only… read more here.

Keywords: arabidopsis thaliana; reverse genetic; arabidopsis; distribution ... See more keywords
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Establishment of a Reverse Genetic System from a Bovine Derived Influenza D Virus Isolate

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recommendations! Published in 2021 at "Viruses"

DOI: 10.3390/v13030502

Abstract: The ruminant-associated influenza D virus (IDV) has a broad host tropism and was shown to have zoonotic potential. To identify and characterize molecular viral determinants influencing the host spectrum of IDV, a reverse genetic system… read more here.

Keywords: isolate; bovine; genetic system; reverse genetic ... See more keywords