Reverse Genetics of Filoviruses.
Sign Up to like & getrecommendations! Published in 2017 at "Current topics in microbiology and immunology"
DOI: 10.1007/82_2017_55
Abstract: Reverse genetics systems are used for the generation of recombinant viruses. For filoviruses, this technology has been available for more than 15 years and has been used to investigate questions regarding the molecular biology, pathogenicity, and… read more here.
Keywords: reverse genetics; genetics systems; genetics; biology ... See more keywords
Plasmid-Based Reverse Genetics of Influenza A Virus.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0346-8_4
Abstract: Reverse genetics is the process of generating an RNA virus from a cDNA copy. Reverse genetics systems have truly transformed our ability to manipulate and study negative-strand RNA viruses. Plasmid-based reverse genetics approaches for influenza… read more here.
Keywords: reverse genetics; genetics; based reverse; plasmid based ... See more keywords
High-throughput screening for negative-stranded hemorrhagic fever viruses using reverse genetics.
Sign Up to like & getrecommendations! Published in 2019 at "Antiviral research"
DOI: 10.1016/j.antiviral.2019.104569
Abstract: Viral hemorrhagic fevers (VHFs) cause thousands of fatalities every year, but the treatment options for their management remain very limited. In particular, the development of therapeutic interventions is restricted by the lack of commercial viability… read more here.
Keywords: reverse genetics; hemorrhagic; genetics; life cycle ... See more keywords
CRISPR/Cas9 screens identify key host factors that enhance rotavirus reverse genetics efficacy and vaccine production
Sign Up to like & getrecommendations! Published in 2024 at "NPJ Vaccines"
DOI: 10.1038/s41541-024-01007-7
Abstract: Rotaviruses pose a significant threat to young children. To identify novel pro- and anti-rotavirus host factors, we performed genome-wide CRISPR/Cas9 screens using rhesus rotavirus and African green monkey cells. Genetic deletion of either SERPINB1 or… read more here.
Keywords: host factors; genetics; rotavirus reverse; reverse genetics ... See more keywords
Reverse Genetics of IRT1, or How to Catch an Iron Transporter and Pin It Down[OPEN]
Sign Up to like & getrecommendations! Published in 2019 at "Plant Cell"
DOI: 10.1105/tpc.19.00246
Abstract: We rely on plants as our main dietary source for the essential micronutrient iron. The Earth’s crust is itself very rich in iron (the fourth most common element by weight), but much of this iron… read more here.
Keywords: reverse genetics; genetics; irt1 catch; genetics irt1 ... See more keywords
Demonstration of bacterium‐free very rapid reverse genetics system using mumps virus
Sign Up to like & getrecommendations! Published in 2022 at "Microbiology and Immunology"
DOI: 10.1111/1348-0421.13032
Abstract: The reverse genetics system is a very powerful tool for analyzing the molecular mechanisms of viral propagation and pathogenesis. However, full‐length genome plasmid construction is highly time‐consuming and laborious, and undesired mutations may be introduced… read more here.
Keywords: mumps virus; using mumps; genetics system; reverse genetics ... See more keywords
Developing reverse genetics systems of northern cereal mosaic virus to reveal superinfection exclusion of two cytorhabdoviruses in barley plants
Sign Up to like & getrecommendations! Published in 2022 at "Molecular Plant Pathology"
DOI: 10.1111/mpp.13188
Abstract: Abstract Recently, reverse genetics systems of plant negative‐stranded RNA (NSR) viruses have been developed to study virus–host interactions. Nonetheless, genetic rescue of plant NSR viruses in both insect vectors and monocot plants is very limited.… read more here.
Keywords: mosaic virus; reverse genetics; superinfection exclusion; genetics ... See more keywords
Establishment of a reverse genetics system for studying human immune functions in mice
Sign Up to like & getrecommendations! Published in 2025 at "Science Advances"
DOI: 10.1126/sciadv.adu1561
Abstract: Reverse genetics approaches in mice are widely used to understand gene functions and their aberrations in diseases. However, limitations exist in translating findings from animal models to human physiology. Humanized mice provide a powerful bridge… read more here.
Keywords: genetics; system; reverse genetics; physiology ... See more keywords
Reverse Genetics System for Heartland Bandavirus: NSs Protein Contributes to Heartland Bandavirus Virulence
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Virology"
DOI: 10.1128/jvi.00049-22
Abstract: Heartland bandavirus (HRTV) is a tick-borne virus identified in the United States in 2009. HRTV causes fever, fatigue, decreased appetite, headache, nausea, diarrhea, and muscle or joint pain in humans. ABSTRACT Heartland bandavirus (HRTV), which… read more here.
Keywords: heartland bandavirus; system; hrtv; reverse genetics ... See more keywords
Plasmid DNA-based reverse genetics as a platform for manufacturing of bluetongue vaccine
Sign Up to like & getrecommendations! Published in 2025 at "Journal of Virology"
DOI: 10.1128/jvi.00139-25
Abstract: ABSTRACT Control of bluetongue disease is predominantly through vaccination with licensed inactivated or live-attenuated vaccines (LAVs). Manufacturing of LAVs in endemic countries requires formulation with a high number of serotypes for effective protection. Herein, we… read more here.
Keywords: genetics; reverse genetics; plasmid dna; genetics platform ... See more keywords
Development of a Mini-Replicon-Based Reverse-Genetics System for Rice Stripe Tenuivirus
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Virology"
DOI: 10.1128/jvi.00589-21
Abstract: Plant-infecting segmented negative-stranded RNA (NSR) viruses are grouped into three genera: Orthotospovirus, Tenuivirus, and Emaravirus. Reverse-genetics systems have been established for members of the genera Orthotospovirus and Emaravirus. ABSTRACT Negative-stranded RNA (NSR) viruses include both… read more here.
Keywords: reverse genetics; mini replicon; system; genetics ... See more keywords