Fragment-Directed Random Mutagenesis by the Reverse Kunkel Method.
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DOI: 10.1021/acssynbio.2c00086
Abstract: Two fundamentally different approaches are routinely used for protein engineering: user-defined mutagenesis and random mutagenesis, each with its own strengths and weaknesses. Here, we invent a unique mutagenesis protocol, which combines the advantages of user-defined… read more here.
Keywords: kunkel method; mutagenesis; random mutagenesis; method ... See more keywords