Electrocatalytic Palladium Nanoclusters as Versatile Indicators of Bioassays: Rapid Electroanalytical Detection of SARS‐CoV‐2 by Reverse Transcription Loop‐Mediated Isothermal Amplification
Sign Up to like & getrecommendations! Published in 2023 at "Advanced Healthcare Materials"
DOI: 10.1002/adhm.202202972
Abstract: Quantitative polymerase chain reaction (qPCR) is considered the gold standard for pathogen detection. However, improvement is still required, especially regarding the possibilities of decentralization. Apart from other reasons, infectious diseases demand on‐site analysis to avoid… read more here.
Keywords: detection sars; transcription loop; detection; loop mediated ... See more keywords
Rapid detection of tilapia lake virus using a one-step reverse transcription loop-mediated isothermal amplification assay
Sign Up to like & getrecommendations! Published in 2019 at "Aquaculture"
DOI: 10.1016/j.aquaculture.2019.04.015
Abstract: Abstract Tilapia lake virus (TiLV) is a newly emerging viral disease in tilapia with recent outbreaks in many parts of the world. There is an urgent need to develop an accurate, sensitive, on-farm method for… read more here.
Keywords: reverse transcription; tilapia lake; lake virus; tilv ... See more keywords
Nuclear Import of the HIV-1 Core Precedes Reverse Transcription and Uncoating
Sign Up to like & getrecommendations! Published in 2020 at "Cell reports"
DOI: 10.1016/j.celrep.2020.108201
Abstract: SUMMARY HIV-1 reverse transcription (RT) occurs before or during uncoating, but the cellular compartment where RT and uncoating occurs is unknown. Using imaging and biochemical assays to track HIV-1 capsids in the nucleus during infection,… read more here.
Keywords: import hiv; hiv; reverse transcription; nuclear import ... See more keywords
Use of a rapid reverse-transcription recombinase aided amplification assay for respiratory syncytial virus detection.
Sign Up to like & getrecommendations! Published in 2018 at "Diagnostic microbiology and infectious disease"
DOI: 10.1016/j.diagmicrobio.2017.10.005
Abstract: In this study, a rapid reverse-transcription recombinase aided amplification (RT-RAA) assay was developed to detect respiratory syncytial virus (RSV) subgroups A and B, respectively. The reaction was performed at 39°C in less than 30min. The… read more here.
Keywords: rapid reverse; recombinase aided; transcription recombinase; aided amplification ... See more keywords
Implication of Pseudo Reference Genes in Normalization of Data from Reverse Transcription-Quantitative PCR.
Sign Up to like & getrecommendations! Published in 2020 at "Gene"
DOI: 10.1016/j.gene.2020.144948
Abstract: Pseudogenes are duplicated or retrotransposed DNA sequences of native functional genes. Amplification of pseudogenes along with gene of interest often produces false positive results, which is an innate problem in Reverse Transcription-quantitative PCR (RT-qPCR). Selecting… read more here.
Keywords: quantitative pcr; transcription quantitative; reference; reverse transcription ... See more keywords
Reverse transcription loop-mediated isothermal amplification for rapid and quantitative assay of covert mortality nodavirus in shrimp
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Invertebrate Pathology"
DOI: 10.1016/j.jip.2015.09.001
Abstract: Abstract A disease known as covert mortality disease has become an increasing problem in the shrimp farming industry in recent years in China and several countries of Southeast Asia, leading to serious losses in production.… read more here.
Keywords: covert mortality; mortality; reverse transcription; shrimp ... See more keywords
MET 14 Skipping Mutation in Pulmonary Sarcomatoid Carcinoma Using Reverse Transcription Polymerase Chain Reaction Method: P117
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Thoracic Oncology"
DOI: 10.1016/j.jtho.2018.10.143
Abstract: Background: Pulmonary sarcomatoid carcinoma (PSC) is a recognized category of highly aggressive and poorly differentiated non-small-cell lung carcinoma (NSCLC), with five different subtypes: pleomorphic, spindle, giant cell, carcinosarcoma, and pulmonary blastoma. Although uncommon (0.1% to… read more here.
Keywords: carcinoma; met skipping; pulmonary sarcomatoid; reverse transcription ... See more keywords
Reverse-transcription loop-mediated isothermal amplification for rapid detection of respiratory syncytial virus directly from nasopharyngeal swabs.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of virological methods"
DOI: 10.1016/j.jviromet.2017.01.006
Abstract: Respiratory syncytial virus (RSV) is a common cause of lower respiratory tract infection in young infants and a major cause of nosocomial infection in pediatric care. Currently available RSV point-of-care tests are of limited sensitivity… read more here.
Keywords: loop mediated; mediated isothermal; respiratory syncytial; syncytial virus ... See more keywords
One-step reverse transcription loop-mediated isothermal amplification assay for detection of Apple chlorotic leaf spot virus.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of virological methods"
DOI: 10.1016/j.jviromet.2017.07.002
Abstract: A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the detection of Apple chlorotic leaf spot virus (ACLSV). In this method, a set of four primers was designed based on the conserved regions… read more here.
Keywords: loop mediated; detection; leaf; reverse transcription ... See more keywords
Rapid detection of two emerging viruses associated with necrotic symptoms in Areca catechu L. by reverse transcription loop-mediated isothermal amplification (RT-LAMP).
Sign Up to like & getrecommendations! Published in 2019 at "Journal of virological methods"
DOI: 10.1016/j.jviromet.2019.113795
Abstract: Two reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assays were developed for the detection of areca palm necrotic ringspot virus (ANRSV) and areca palm necrotic spindle-spot virus (ANSSV), respectively. These two emerging viruses both induce necrotic symptoms… read more here.
Keywords: loop mediated; lamp; detection; reverse transcription ... See more keywords
A closed-tube, single-step, real time, reverse transcription-loop-mediated isothermal amplification assay for infectious bronchitis virus detection in chickens
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Virological Methods"
DOI: 10.1016/j.jviromet.2020.113940
Abstract: Abstract Infectious bronchitis (IB) is a viral infection of the chicken respiratory tract that causes substantial economic burden on the industry. Simple, specific and rapid diagnosis of this disease is critical for the initiation of… read more here.
Keywords: reverse transcription; detection; tube; infectious bronchitis ... See more keywords