Chiral separation of dipeptides on Cinchona-based zwitterionic chiral stationary phases under buffer-free reversed-phase conditions.
Sign Up to like & getrecommendations! Published in 2022 at "Chirality"
DOI: 10.1002/chir.23471
Abstract: Chiral zwitterion ion exchangers represent efficient chiral stationary phases for stereoselective resolution of various analytes including chiral acids, bases, and zwitterions. In this contribution, we have focused on utilization of chiral zwitterionic sorbents, denoted as… read more here.
Keywords: reversed phase; stationary phases; free reversed; buffer free ... See more keywords
Comprehensive metabonomic analysis of heart tissue from isoproterenol-induced myocardial infarction rat based on reversed-phase and hydrophilic interaction chromatography coupled to mass spectrometry.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of separation science"
DOI: 10.1002/jssc.201601013
Abstract: We aim to describe the metabonomic characteristics of myocardial infarction rats. High-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was utilized to develop a metabonomic method of the heart homogenates of myocardial infarction rats.… read more here.
Keywords: chromatography; reversed phase; heart; myocardial infarction ... See more keywords
Preparative isolation of 1, 1-diphenyl-2-picrylhydrazyl inhibitors from Ribes himalense using medium-pressure and two-dimensional reversed-phase/reversed-phase liquid chromatography guided by an online HPLC-1, 1-diphenyl-2-picrylhydrazyl assay.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of separation science"
DOI: 10.1002/jssc.202001080
Abstract: The lack of suitable chromatographic purification methods makes it a challenge to effectively isolate the chemical components of traditional Tibetan medicines. Ribes himalense is a rarely studied Tibetan medicine, reputed to have free radical-scavenging effects.… read more here.
Keywords: himalense; reversed phase; diphenyl picrylhydrazyl;
A novel quality by design approach for development and validation of a green reversed-phase HPLC method with fluorescence detection for the simultaneous determination of lesinurad, febuxostat, and diflunisal: Application to human plasma.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of separation science"
DOI: 10.1002/jssc.202100016
Abstract: A novel and eco-friendly reversed-phase HPLC method with fluorescence detection was developed for simultaneous estimation of two co-administered antigout drugs (lesinurad and febuxostat) with diflunisal as a non-steroidal anti-inflammatory drug. Unlike routine methodology, the developed… read more here.
Keywords: lesinurad febuxostat; reversed phase; method; design ... See more keywords
OFF-line two-dimensional liquid chromatography separation for the quality control of saponins samples from Quillaja Saponaria.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of separation science"
DOI: 10.1002/jssc.202100115
Abstract: Quil-A is a purified extract of saponins with strong immunoadjuvant activity. While specific molecules have been identified and tested in clinical trials, Quil-A is mostly used as a totum of the Quillaja Saponaria bark extract.… read more here.
Keywords: chromatography; phase; reversed phase; quillaja saponaria ... See more keywords
Reversed-Phase HPLC: A fast and efficient analytical method to quantify docetaxel-loaded pegylated liposomes in release study.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of separation science"
DOI: 10.1002/jssc.202100382
Abstract: Docetaxel is an anticancer that belongs to the family of taxanes and acts in the inhibition of cell proliferation through the polymerization of microtubules. The aim of this study was the development and validation a… read more here.
Keywords: hplc; analytical method; reversed phase; pegylated liposomes ... See more keywords
Effect of mobile-phase modifiers on the enantioselective retention behavior of methyl mandelate with an amylose 3,5-dimethylphenylcarbamate chiral stationary phase under reversed-phase conditions.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of separation science"
DOI: 10.1002/jssc.202200651
Abstract: In this study, methanol, ethanol, n-propyl alcohol, isopropyl alcohol, acetone, and tert-butanol were used as organic modifiers in reversed-phase mode chiral liquid-chromatography to systematically investigate the effects of mobile phase components on the enantioselective retention… read more here.
Keywords: retention; reversed phase; retention behavior; phase ... See more keywords
Prediction of peptides retention behavior in reversed‐phase liquid chromatography based on their hydrophobicity
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Separation Science"
DOI: 10.1002/jssc.202200743
Abstract: Hydrophobicity is an important physicochemical property of peptides and proteins. It is responsible for their conformational changes, stability, as well as various chemical intramolecular and intermolecular interactions. Enormous efforts have been invested to study the… read more here.
Keywords: liquid chromatography; hydrophobicity; phase liquid; reversed phase ... See more keywords
Quantification of In Vitro Protein Lysine Acetylation by Reversed Phase HPLC.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9434-2_4
Abstract: Protein lysine acetylation is a reversible posttranslational modification that is catalyzed by a group of enzymes that are collectively referred to as lysine (K) acetyltransferases (KATs). These enzymes catalyze the transfer of the acetyl group… read more here.
Keywords: reversed phase; phase hplc; protein lysine; lysine acetylation ... See more keywords
High-selectivity profiling of released and labeled N-glycans via polar-embedded reversed-phase chromatography
Sign Up to like & getrecommendations! Published in 2018 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-018-1495-7
Abstract: AbstractN-Glycosylation is the most complex post-translational modification of proteins and involved in many physiological processes and is therefore of major interest in academic research and in the biopharmaceutical industry. Reliable, robust, reproducible, and selective analysis… read more here.
Keywords: chromatography; phase; reversed phase; polar embedded ... See more keywords
Retention dependences support highly confident identification of lipid species in human plasma by reversed-phase UHPLC/MS
Sign Up to like & getrecommendations! Published in 2021 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-021-03492-4
Abstract: Reversed-phase ultrahigh-performance liquid chromatography-mass spectrometry (RP-UHPLC/MS) method was developed with the aim to unambiguously identify a large number of lipid species from multiple lipid classes in human plasma. The optimized RP-UHPLC/MS method employed the C18… read more here.
Keywords: reversed phase; human plasma; mass; identification ... See more keywords