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   Articles with "rhamnosidase" as a keyword



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Homologous Expression and Characterization of α-L-rhamnosidase from Aspergillus niger for the Transformation of Flavonoids

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recommendations! Published in 2022 at "Applied Biochemistry and Biotechnology"

DOI: 10.1007/s12010-022-03894-9

Abstract: Aspergillus niger has been used for homologous and heterologous expressions of many protein products. In this study, the α-L-rhamnosidase from A. niger (Rha-N1, GenBank XP_001389086.1) was homologously expressed in A. niger 3.350 by Agrobacterium tumefaciens-mediated… read more here.

Keywords: aspergillus niger; characterization rhamnosidase; homologous expression; transformation ... See more keywords
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Structural and functional insights into RHA-P, a bacterial GH106 α-L-rhamnosidase from Novosphingobium sp. PP1Y.

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recommendations! Published in 2018 at "Archives of biochemistry and biophysics"

DOI: 10.1016/j.abb.2018.04.013

Abstract: α-L-Rhamnosidases (α-RHAs, EC 3.2.1.40) are glycosyl hydrolases (GHs) hydrolyzing terminal α-l-rhamnose residues from different substrates such as heteropolysaccharides, glycosylated proteins and natural flavonoids. Although the possibility to hydrolyze rhamnose from natural flavonoids has boosted the… read more here.

Keywords: natural flavonoids; functional insights; novosphingobium pp1y; structural functional ... See more keywords

Characterization of a α-l-rhamnosidase from Bacteroides thetaiotaomicron with high catalytic efficiency of epimedin C.

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recommendations! Published in 2018 at "Bioorganic chemistry"

DOI: 10.1016/j.bioorg.2018.08.004

Abstract: In this study, a α-l-rhamnosidase gene from Bacteroides thetaiotaomicron VPI-5482 was cloned and expressed in Escherichia coli. The specific activity of rhamnosidase was 0.57 U/mg in LB medium with 0.1 mM Isopropyl β-d-Thiogalactoside (IPTG) induction at… read more here.

Keywords: bacteroides thetaiotaomicron; rhamnoside rhamnoside; epimedin; rhamnosidase ... See more keywords
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Molecular insights into catalytic specificity of α-L-rhamnosidase from Bacteroides thetaiotaomicron by molecular docking and dynamics

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recommendations! Published in 2020 at "Chemical Physics Letters"

DOI: 10.1016/j.cplett.2020.137695

Abstract: Abstract Recently, a kind of α-L-rhamnosidase from Bacteroides thetaiotaomicron (BtRha) was characterized and studied for the hydrolysis of natural substrates. In order to investigate catalytic specificity of BtRha, three natural substrates, epimedin C, naringin, and… read more here.

Keywords: bacteroides thetaiotaomicron; docking dynamics; catalytic specificity; rhamnosidase ... See more keywords

B-factor-saturation mutagenesis as a strategy to increase the thermostability of α-L-rhamnosidase from Aspergillus terreus.

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recommendations! Published in 2018 at "Journal of biotechnology"

DOI: 10.1016/j.jbiotec.2018.03.013

Abstract: The α-L-Rhamnosidase is an important enzyme with applications in the food and pharmaceutical industries because it can release terminal L-rhamnose residues from various natural products. In this study, the B-factor-saturation mutagenesis strategy was used to… read more here.

Keywords: d594q g827k; g827k d594q; thermostability; d594q mutant ... See more keywords
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Heterologous Expression and Characterization of a New Clade of Aspergillus α-L-Rhamnosidase Suitable for Citrus Juice Processing.

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recommendations! Published in 2019 at "Journal of agricultural and food chemistry"

DOI: 10.1021/acs.jafc.8b06932

Abstract: α-L-Rhamnosidase is a glycoside hydrolase capable of removing naringin from citrus juice. However, α-L-rhamnosidases always have broad substrate spectra, causing negative effects on citrus juice. In this study, a α-L-rhamnosidase-expressing fungal strain, JMU-TS529, was identified,… read more here.

Keywords: clade aspergillus; new clade; rhamnosidase; citrus juice ... See more keywords
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Purification and Characterization of a Novel α-L-Rhamnosidase from Papiliotrema laurentii ZJU-L07 and Its Application in Production of Icariin from Epimedin C

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recommendations! Published in 2022 at "Journal of Fungi"

DOI: 10.3390/jof8060644

Abstract: Icariin is the most effective bioactive compound in Herba Epimedii. To enhance the content of icariin in the epimedium water extract, a novel strain, Papiliotrema laurentii ZJU-L07, producing an intracellular α-L-rhamnosidase was isolated from the… read more here.

Keywords: rhamnosidase; icariin; laurentii zju; production icariin ... See more keywords
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The Loss-of-Function Mutation aldA67 Leads to Enhanced α-L-Rhamnosidase Production by Aspergillus nidulans

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recommendations! Published in 2022 at "Journal of Fungi"

DOI: 10.3390/jof8111181

Abstract: In Aspergillus nidulans L-rhamnose is catabolised to pyruvate and L-lactaldehyde, and the latter ultimately to L-lactate, via the non-phosphorylated pathway (LRA) encoded by the genes lraA-D, and aldA that encodes a broad substrate range aldehyde… read more here.

Keywords: rhamnosidase; loss function; loss; production ... See more keywords