Agrobacterium rhizogenes-mediated RNAi of Tripterygium wilfordii and application for functional study of terpenoid biosynthesis pathway genes
Sign Up to like & getrecommendations! Published in 2019 at "Industrial Crops and Products"
DOI: 10.1016/j.indcrop.2019.111509
Abstract: Abstract Tripterygium wilfordii is an important medical plants which is rich in several valuable bioactive terpenoids, such as triptolide and wilforine. Developing a fast and efficient transformation method is imperative for exploring these metabolites biosynthesis… read more here.
Keywords: biosynthesis pathway; tripterygium; tripterygium wilfordii; agrobacterium rhizogenes ... See more keywords
Use of the rhizobial type III effector gene nopP to improve Agrobacterium rhizogenes-mediated transformation of Lotus japonicus
Sign Up to like & getrecommendations! Published in 2021 at "Plant Methods"
DOI: 10.1186/s13007-021-00764-z
Abstract: Background Protocols for Agrobacterium rhizogenes -mediated hairy root transformation of the model legume Lotus japonicus have been established previously. However, little efforts were made in the past to quantify and improve the transformation efficiency. Here,… read more here.
Keywords: lotus japonicus; transformation; agrobacterium rhizogenes; japonicus ... See more keywords
AtGCS promoter-driven clustered regularly interspaced short palindromic repeats/Cas9 highly efficiently generates homozygous/biallelic mutations in the transformed roots by Agrobacterium rhizogenes–mediated transformation
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Plant Science"
DOI: 10.3389/fpls.2022.952428
Abstract: Agrobacterium rhizogenes–mediated (ARM) transformation is an efficient and powerful tool to generate transgenic roots to study root-related biology. For loss-of-function studies, transgenic-root-induced indel mutations by CRISPR/Cas9 only with homozygous/biallelic mutagenesis can exhibit mutant phenotype(s) (excluding… read more here.
Keywords: promoter; homozygous biallelic; rhizogenes mediated; agrobacterium rhizogenes ... See more keywords