Efficient genome editing in Claviceps purpurea using a CRISPR/Cas9 ribonucleoprotein method
Sign Up to like & getrecommendations! Published in 2022 at "Synthetic and Systems Biotechnology"
DOI: 10.1016/j.synbio.2022.02.002
Abstract: Claviceps purpurea produces many pharmacologically important ergot alkaloids (EAS), which are widely used to treat migraine and hypertension and to aid childbirth. Although an EAS biosynthetic cluster of C. purpurea has been discovered more than… read more here.
Keywords: crispr cas9; efficient genome; ribonucleoprotein; genome editing ... See more keywords
Elucidation of structure–function relationships in Methanocaldococcus jannaschii RNase P, a multi-subunit catalytic ribonucleoprotein
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac595
Abstract: Abstract RNase P is a ribonucleoprotein (RNP) that catalyzes removal of the 5′ leader from precursor tRNAs in all domains of life. A recent cryo-EM study of Methanocaldococcus jannaschii (Mja) RNase P produced a model… read more here.
Keywords: elucidation structure; structure function; methanocaldococcus jannaschii; rnase ... See more keywords
Isolation of Reconstructed Functional Ribonucleoprotein Complexes of Machupo Virus
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Virology"
DOI: 10.1128/jvi.01054-21
Abstract: Arenaviruses are rodent-borne pathogens that can cause severe disease in humans. All arenaviruses begin the infection cycle with delivery of the virus replication machinery into the cytoplasm of the host cell. ABSTRACT Arenaviruses initiate infection… read more here.
Keywords: machupo virus; ribonucleoprotein; replication; machinery ... See more keywords
Knocking out consumer concerns and regulator’s rules: efficient use of CRISPR/Cas ribonucleoprotein complexes for genome editing in cereals
Sign Up to like & getrecommendations! Published in 2017 at "Genome Biology"
DOI: 10.1186/s13059-017-1179-1
Abstract: Selection-free genome editing using Cas9 ribonucleoprotein embryo bombardment has been achieved for maize and wheat. This is a breakthrough that should make new breeding technologies more acceptable for worldwide use. read more here.
Keywords: concerns regulator; genome editing; use; ribonucleoprotein ... See more keywords
The β-1,3-Glucanase Degrades Callose at Plasmodesmata to Facilitate the Transport of the Ribonucleoprotein Complex in Pyrus betulaefolia
Sign Up to like & getrecommendations! Published in 2023 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms24098051
Abstract: Grafting is widely used to improve the stress tolerance and the fruit yield of horticultural crops. Ribonucleoprotein complexes formed by mRNAs and proteins play critical roles in the communication between scions and stocks of grafted… read more here.
Keywords: transport ribonucleoprotein; pyrus betulaefolia; glucanase; ribonucleoprotein ... See more keywords
Components and Architecture of the Rhabdovirus Ribonucleoprotein Complex
Sign Up to like & getrecommendations! Published in 2020 at "Viruses"
DOI: 10.3390/v12090959
Abstract: Rhabdoviruses, as single-stranded, negative-sense RNA viruses within the order Mononegavirales, are characterised by bullet-shaped or bacteroid particles that contain a helical ribonucleoprotein complex (RNP). Here, we review the components of the RNP and its higher-order… read more here.
Keywords: ribonucleoprotein complex; rhabdovirus ribonucleoprotein; components architecture; ribonucleoprotein ... See more keywords