An optimized yeast display strategy for efficient scFv antibody selection using ribosomal skipping system and thermo resistant yeast
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology Letters"
DOI: 10.1007/s10529-019-02710-5
Abstract: ObjectivesEstablish a method to restrict unexpected fragments including stop codons in scFv library and generate a thermo resistant strain for screening of thermal stable scFv sequences.ResultsHere, we have constructed a T2A–Leu2 system for selection of… read more here.
Keywords: ribosomal skipping; thermo resistant; resistant yeast; selection ... See more keywords
Inefficient Ribosomal Skipping Enables Simultaneous Secretion and Display of Proteins in Saccharomyces cerevisiae.
Sign Up to like & getrecommendations! Published in 2017 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.7b00144
Abstract: The need for recombinant expression of soluble protein slows the validation of engineered proteins isolated from combinatorial libraries and limits the number of protein variants evaluated. To overcome this bottleneck, we describe a system for… read more here.
Keywords: protein; ribosomal skipping; secretion; display ... See more keywords