Nuclear export of the pre-60S ribosomal subunit through single nuclear pores observed in real time
Sign Up to like & getrecommendations! Published in 2021 at "Nature Communications"
DOI: 10.1038/s41467-021-26323-7
Abstract: Ribosomal biogenesis has been studied by biochemical, genetic and electron microscopic approaches, but live cell data on the in vivo kinetics are still missing. Here we analyse the export kinetics of the large ribosomal subunit… read more here.
Keywords: export; nuclear export; ribosomal subunit; pre 60s ... See more keywords
In vitro reconstitution of functional small ribosomal subunit assembly for comprehensive analysis of ribosomal elements in E. coli
Sign Up to like & getrecommendations! Published in 2020 at "Communications Biology"
DOI: 10.1038/s42003-020-0874-8
Abstract: In vitro reconstitution is a powerful tool for investigating ribosome functions and biogenesis, as well as discovering new ribosomal features. In this study, we integrated all of the processes required for Escherichia coli small ribosomal… read more here.
Keywords: ribosomal subunit; subunit assembly; small ribosomal; subunit ... See more keywords
The context of the ribosome binding site in mRNAs defines specificity of action of kasugamycin, an inhibitor of translation initiation
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2118553119
Abstract: Significance Several antibiotics targeting the large ribosomal subunit interfere with translation in a context-specific manner, preventing ribosomes from polymerizing specific amino acid sequences. Here, we reveal kasugamycin as a small ribosomal subunit-targeting antibiotic whose action… read more here.
Keywords: specificity action; ribosomal subunit; action; context ... See more keywords
Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunit
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac1266
Abstract: Assembly of ribosomal subunits into active ribosomal complexes is integral to protein synthesis. Release of eIF6 from the 60S ribosomal subunit primes 60S to associate with the 40S subunit and engage in translation. The dynamics… read more here.
Keywords: ribosomal subunit; 60s ribosomal; interface; dynamic states ... See more keywords
The DEAH-box RNA helicase Dhr1 contains a remarkable carboxyl terminal domain essential for small ribosomal subunit biogenesis
Sign Up to like & getrecommendations! Published in 2019 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkz529
Abstract: Abstract Ribosome biogenesis is an essential process in all living cells, which entails countless highly sequential and dynamic structural reorganization events. These include formation of dozens RNA helices through Watson-Crick base-pairing within ribosomal RNAs (rRNAs)… read more here.
Keywords: dhr1; ribosomal subunit; deah box; small ribosomal ... See more keywords
Pol5 is an essential ribosome biogenesis factor required for 60S ribosomal subunit maturation in Saccharomyces cerevisiae.
Sign Up to like & getrecommendations! Published in 2019 at "RNA"
DOI: 10.1261/rna.072116.119
Abstract: In Saccharomyces cerevisiae more than 250 trans-acting factors are involved in the maturation of 40S and 60S ribosomal subunits. The expression of most of these factors is transcriptionally co-regulated to ensure correct ribosome production under… read more here.
Keywords: 60s ribosomal; saccharomyces cerevisiae; ribosomal subunit; factor required ... See more keywords
The Npa1p complex chaperones the assembly of the earliest eukaryotic large ribosomal subunit precursor
Sign Up to like & getrecommendations! Published in 2018 at "PLoS Genetics"
DOI: 10.1371/journal.pgen.1007597
Abstract: The early steps of the production of the large ribosomal subunit are probably the least understood stages of eukaryotic ribosome biogenesis. The first specific precursor to the yeast large ribosomal subunit, the first pre-60S particle,… read more here.
Keywords: pre ribosomal; ribosomal subunit; large ribosomal; 25s rrna ... See more keywords
Molecular functions of RNA helicases during ribosomal subunit assembly
Sign Up to like & getrecommendations! Published in 2023 at "Biological Chemistry"
DOI: 10.1515/hsz-2023-0135
Abstract: Abstract During their biogenesis, the ribosomal subunits undergo numerous structural and compositional changes to achieve their final architecture. RNA helicases are a key driving force of such remodelling events but deciphering their particular functions has… read more here.
Keywords: rna helicases; molecular functions; ribosomal subunit; functions rna ... See more keywords
Overexpressed L20 Rescues 50S Ribosomal Subunit Assembly Defects of bipA-Deletion in Escherichia coli
Sign Up to like & getrecommendations! Published in 2019 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2019.02982
Abstract: The BipA (BPI-inducible protein A) protein is highly conserved in a large variety of bacteria and belongs to the translational GTPases, based on sequential and structural similarities. Despite its conservation in bacteria, bipA is not… read more here.
Keywords: ribosomal subunit; bipa; assembly; 50s ribosomal ... See more keywords
The Terminal Extensions of Dbp7 Influence Growth and 60S Ribosomal Subunit Biogenesis in Saccharomyces cerevisiae
Sign Up to like & getrecommendations! Published in 2023 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms24043460
Abstract: Ribosome synthesis is a complex process that involves a large set of protein trans-acting factors, among them DEx(D/H)-box helicases. These are enzymes that carry out remodelling activities onto RNAs by hydrolysing ATP. The nucleolar DEGD-box… read more here.
Keywords: growth 60s; ribosomal subunit; terminal extensions; biogenesis ... See more keywords