Determination of the ribosome structure to a resolution of 2.5 Å by single‐particle cryo‐EM
Sign Up to like & getrecommendations! Published in 2017 at "Protein Science"
DOI: 10.1002/pro.3068
Abstract: With the advance of new instruments and algorithms, and the accumulation of experience over decades, single‐particle cryo‐EM has become a pivotal part of structural biology. Recently, we determined the structure of a eukaryotic ribosome at… read more here.
Keywords: ribosome; resolution; single particle; particle cryo ... See more keywords
mQC: A post-mapping data exploration tool for ribosome profiling
Sign Up to like & getrecommendations! Published in 2019 at "Computer methods and programs in biomedicine"
DOI: 10.1016/j.cmpb.2018.10.018
Abstract: BACKGROUND AND OBJECTIVE Ribosome profiling is a recent next generation sequencing technique enabling the genome-wide study of gene expression in biomedical research at the translation level. Too often, researchers precipitously start trying to test their… read more here.
Keywords: profiling data; ribosome profiling; exploration; tool ... See more keywords
Unique structural features of the Mycobacterium ribosome.
Sign Up to like & getrecommendations! Published in 2019 at "Progress in biophysics and molecular biology"
DOI: 10.1016/j.pbiomolbio.2019.12.001
Abstract: Protein synthesis in all the living cells is mediated by a large protein-RNA complex called the ribosome. These macromolecular complexes can range from 2.5 (prokaryotes) to 4.2 MDa. (eukaryotes) in size and undergo various conformational transitions… read more here.
Keywords: unique structural; features mycobacterium; mycobacterium ribosome; structural features ... See more keywords
Ribosome Assembly in Trypanosomatids: A Novel Therapeutic Target.
Sign Up to like & getrecommendations! Published in 2017 at "Trends in parasitology"
DOI: 10.1016/j.pt.2016.12.003
Abstract: In Liu et al., the authors present a 2.5-Å structure of the Trypanosoma cruzi 60S ribosomal subunit and propose a model for the stepwise assembly of the large-subunit ribosomal RNA (rRNA). Based on this study,… read more here.
Keywords: ribosome assembly; assembly trypanosomatids; trypanosomatids novel; ribosome ... See more keywords
Thio-Modification of tRNA at the Wobble Position as Regulator of the Kinetics of Decoding and Translocation on the Ribosome.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.7b00727
Abstract: Uridine 34 (U34) at the wobble position of the tRNA anticodon is post-transcriptionally modified, usually to mcm5s2, mcm5, or mnm5. The lack of the mcm5 or s2 modification at U34 of tRNALys, tRNAGlu, and tRNAGln… read more here.
Keywords: trnalys; translocation; wobble position; ribosome ... See more keywords
Engineered ribosomes with tethered subunits for expanding biological function
Sign Up to like & getrecommendations! Published in 2019 at "Nature Communications"
DOI: 10.1038/s41467-019-11427-y
Abstract: Ribo-T is a ribosome with covalently tethered subunits where core 16S and 23S ribosomal RNAs form a single chimeric molecule. Ribo-T makes possible a functionally orthogonal ribosome–mRNA system in cells. Unfortunately, use of Ribo-T has… read more here.
Keywords: tethered subunits; protein expression; engineered ribosomes; ribosomes tethered ... See more keywords
Human NMD ensues independently of stable ribosome stalling
Sign Up to like & getrecommendations! Published in 2020 at "Nature Communications"
DOI: 10.1038/s41467-020-17974-z
Abstract: Nonsense-mediated mRNA decay (NMD) is a translation-dependent RNA degradation pathway that is important for the elimination of faulty, and the regulation of normal, mRNAs. The molecular details of the early steps in NMD are not… read more here.
Keywords: ribosome stalling; nmd ensues; nmd sensitive; ribosome ... See more keywords
Structure of Pseudomonas aeruginosa ribosomes from an aminoglycoside-resistant clinical isolate
Sign Up to like & getrecommendations! Published in 2019 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.1909831116
Abstract: Significance The cryo-electron microscopy high-resolution structures of the wild-type ribosome of the human pathogen Pseudomonas aeruginosa and its uL6 rProtein mutant, isolated from a cystic fibrosis (CF) patient, shed light on the link between a… read more here.
Keywords: pseudomonas aeruginosa; initiation; microscopy; binding site ... See more keywords
Translation initiation site of mRNA is selected through dynamic interaction with the ribosome
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2118099119
Abstract: Significance Ribosomes translate the genetic codes of messenger RNA (mRNA) to make proteins. Translation must begin at the correct initiation site; otherwise, abnormal proteins will be produced. Here, we show that a short ribosome-specific sequence… read more here.
Keywords: initiation site; initiation; site; translation initiation ... See more keywords
Development of a ribosome profiling protocol to study translation in Kluyveromyces marxianus
Sign Up to like & getrecommendations! Published in 2022 at "FEMS Yeast Research"
DOI: 10.1093/femsyr/foac024
Abstract: Abstract Kluyveromyces marxianus is an interesting and important yeast because of particular traits such as thermotolerance and rapid growth, and for applications in food and industrial biotechnology. For both understanding its biology and developing bioprocesses,… read more here.
Keywords: development ribosome; kluyveromyces marxianus; ribosome profiling; translation ... See more keywords
Rare ribosomal RNA sequences from archaea stabilize the bacterial ribosome
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac1273
Abstract: Ribosomes serve as the universally conserved translators of the genetic code into proteins and must support life across temperatures ranging from below freezing to above the boiling point of water. Ribosomes are capable of functioning… read more here.
Keywords: ribosomal rna; bacterial ribosome; transferase center; peptidyl transferase ... See more keywords