Selection of an artificial paraquat-specific binding protein from a ribosome display library based on a lipocalin scaffold.
Sign Up to like & getrecommendations! Published in 2020 at "Biotechnology and applied biochemistry"
DOI: 10.1002/bab.2059
Abstract: In this study, an anticalin that could specifically bind paraquat (PQ), a quaternary nitrogen herbicide, as a new set of engineered receptor protein with antibody-like properties was generated to detect PQ concentration. To this end,… read more here.
Keywords: library; selection artificial; ribosome display; binding protein ... See more keywords
Combination of ribosome display and next generation sequencing as a powerful method for identification of affibody binders against β-lactamase CTX-M15.
Sign Up to like & getrecommendations! Published in 2019 at "New biotechnology"
DOI: 10.1016/j.nbt.2019.01.004
Abstract: CTX-M15 is one of the most widespread, extended spectrum β-lactamases, a major determinant of antibiotic resistance representing urgent public health threats, among enterobacterial strains infecting humans and animals. Here we describe the selection of binders… read more here.
Keywords: ctx m15; next generation; affibody; ribosome display ... See more keywords
Ribosome display for the rapid generation of high-affinity Zika-neutralizing single-chain antibodies
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DOI: 10.1371/journal.pone.0205743
Abstract: Background Zika virus (ZIKV) is an emerging pathogen with no approved therapeutics and only limited diagnostics available. To address this gap, six mouse single-chain antibodies (scFvs) to ZIKV envelope (E) protein were isolated rapidly and… read more here.
Keywords: affinity; zikv; chain antibodies; scfvs ... See more keywords