Ribosome stalling caused by the Argonaute-microRNA-SGS3 complex regulates the production of secondary siRNAs in plants.
Sign Up to like & getrecommendations! Published in 2021 at "Cell reports"
DOI: 10.1016/j.celrep.2021.109300
Abstract: The path of ribosomes on mRNAs can be impeded by various obstacles. One such example is halting of ribosome movement by microRNAs, but the exact mechanism and physiological role remain unclear. Here, we find that… read more here.
Keywords: caused argonaute; ribosome stalling; stalling caused; production secondary ... See more keywords
Live-cell imaging reveals kinetic determinants of quality control triggered by ribosome stalling.
Sign Up to like & getrecommendations! Published in 2021 at "Molecular cell"
DOI: 10.1016/j.molcel.2021.01.029
Abstract: Translation of problematic mRNA sequences induces ribosome stalling, triggering quality-control events, including ribosome rescue and nascent polypeptide degradation. To define the timing and regulation of these processes, we developed a SunTag-based reporter to monitor translation… read more here.
Keywords: quality control; control; ribosome stalling; live cell ... See more keywords
Ribosome Stalling of N-Linked Glycoproteins in Cell-Free Extracts
Sign Up to like & getrecommendations! Published in 2022 at "ACS Synthetic Biology"
DOI: 10.1021/acssynbio.2c00311
Abstract: Ribosome display is a powerful in vitro method for selection and directed evolution of proteins expressed from combinatorial libraries. However, the ability to display proteins with complex post-translational modifications such as glycosylation is limited. To… read more here.
Keywords: cell free; ribosome stalling; stalling linked; linked glycoproteins ... See more keywords
Human NMD ensues independently of stable ribosome stalling
Sign Up to like & getrecommendations! Published in 2020 at "Nature Communications"
DOI: 10.1038/s41467-020-17974-z
Abstract: Nonsense-mediated mRNA decay (NMD) is a translation-dependent RNA degradation pathway that is important for the elimination of faulty, and the regulation of normal, mRNAs. The molecular details of the early steps in NMD are not… read more here.
Keywords: ribosome stalling; nmd ensues; nmd sensitive; ribosome ... See more keywords
Critical 23S rRNA interactions for macrolide-dependent ribosome stalling on the ErmCL nascent peptide chain
Sign Up to like & getrecommendations! Published in 2017 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkx195
Abstract: Abstract The nascent peptide exit tunnel has recently been identified as a functional region of ribosomes contributing to translation regulation and co-translational protein folding. Inducible expression of the erm resistance genes depends on ribosome stalling… read more here.
Keywords: ribosome stalling; exit tunnel; peptide; nascent peptide ... See more keywords
Loss of N1-methylation of G37 in tRNA induces ribosome stalling and reprograms gene expression
Sign Up to like & getrecommendations! Published in 2021 at "eLife"
DOI: 10.7554/elife.70619
Abstract: N1-methylation of G37 is required for a subset of tRNAs to maintain the translational reading-frame. While loss of m1G37 increases ribosomal +1 frameshifting, whether it incurs additional translational defects is unknown. Here, we address this… read more here.
Keywords: m1g37 deficiency; methylation g37; ribosome stalling; deficiency ... See more keywords