Investigation of Mitochondrial ADP-Ribosylation Via Immunofluorescence.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1266-8_12
Abstract: ADP-ribosylation is a posttranslational protein modification, involved in various cellular processes, ranging from DNA-damage repair to apoptosis. While its function has been studied amply with respect to genotoxic stress-associated nuclear ADP-ribosylation, the functional relevance of… read more here.
Keywords: ribosylation via; mitochondrial adp; adp ribosylation; ribosylation ... See more keywords
Ribosylation induced structural changes in Bovine Serum Albumin: understanding high dietary sugar induced protein aggregation and amyloid formation
Sign Up to like & getrecommendations! Published in 2020 at "Heliyon"
DOI: 10.1016/j.heliyon.2020.e05053
Abstract: Non-enzymatic glycation of proteins is believed to be the root cause of high dietary sugar associated pathophysiological maladies. We investigated the structural changes in protein during progression of glycation using ribosylated Bovine Serum Albumin (BSA).… read more here.
Keywords: formation; ribosylation; glycation; dietary sugar ... See more keywords
Development of an ADP-ribosylation assay for residual toxicity in C. difficile binary toxin CDTa using automated capillary western blot.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of pharmaceutical and biomedical analysis"
DOI: 10.1016/j.jpba.2020.113125
Abstract: CDTa, an actin ADP-ribosylation transferase, is a binary toxin produced by the bacterium Clostridium difficile which is commonly associated with the hypervirulent strain present in Clostridium difficile infections. The mutated form of CDTa, 4mCDTa, is… read more here.
Keywords: binary toxin; difficile; adp ribosylation; residual toxicity ... See more keywords
A Protein Semisynthesis-Based Strategy to Investigate the Functional Impact of Linker Histone Serine ADP-Ribosylation.
Sign Up to like & getrecommendations! Published in 2022 at "ACS chemical biology"
DOI: 10.1021/acschembio.2c00091
Abstract: Recently developed chemical and enzyme-based technologies to install serine ADP-ribosylation onto synthetic peptides have enabled new approaches to study poly(ADP-ribose) polymerase (PARP) biology. Here, we establish a generalizable strategy to prepare ADP-ribosylated peptides that are… read more here.
Keywords: adp ribosylation; ribosylation; linker histone; biology ... See more keywords
An Integrated Chemical Proteomics Approach for Quantitative Profiling of Intracellular ADP-Ribosylation
Sign Up to like & getrecommendations! Published in 2019 at "Scientific Reports"
DOI: 10.1038/s41598-019-43154-1
Abstract: ADP-ribosylation is integral to a diverse range of cellular processes such as DNA repair, chromatin regulation and RNA processing. However, proteome-wide investigation of its cellular functions has been limited due to numerous technical challenges including… read more here.
Keywords: integrated chemical; ribosylation; mass spectrometry; adp ribosylation ... See more keywords
Beyond protein modification: the rise of non-canonical ADP-ribosylation
Sign Up to like & getrecommendations! Published in 2022 at "Biochemical Journal"
DOI: 10.1042/bcj20210280
Abstract: ADP-ribosylation has primarily been known as post-translational modification of proteins. As signalling strategy conserved in all domains of life, it modulates substrate activity, localisation, stability or interactions, thereby regulating a variety of cellular processes and… read more here.
Keywords: modification; adp ribosylation; canonical adp; non canonical ... See more keywords
The function and regulation of ADP-ribosylation in the DNA damage response.
Sign Up to like & getrecommendations! Published in 2023 at "Biochemical Society transactions"
DOI: 10.1042/bst20220749
Abstract: ADP-ribosylation is a post-translational modification involved in DNA damage response (DDR). In higher organisms it is synthesised by PARP 1-3, DNA strand break sensors. Recent advances have identified serine residues as the most common targets… read more here.
Keywords: dna damage; adp ribosylation; ribosylation; damage response ... See more keywords
ADPriboDB 2.0: an updated database of ADP-ribosylated proteins
Sign Up to like & getrecommendations! Published in 2021 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkaa941
Abstract: Abstract ADP-ribosylation is a protein modification responsible for biological processes such as DNA repair, RNA regulation, cell cycle and biomolecular condensate formation. Dysregulation of ADP-ribosylation is implicated in cancer, neurodegeneration and viral infection. We developed… read more here.
Keywords: adp ribosylated; adp ribosylation; ribosylated proteins; database ... See more keywords
Structural insights into ADP-ribosylation of ubiquitin by Deltex family E3 ubiquitin ligases
Sign Up to like & getrecommendations! Published in 2020 at "Science Advances"
DOI: 10.1126/sciadv.abc0418
Abstract: The C-terminal RING and DTC domains of mammalian Deltex proteins catalyze ADP-ribosylation at the C terminus of ubiquitin. Cellular cross-talk between ubiquitination and other posttranslational modifications contributes to the regulation of numerous processes. One example… read more here.
Keywords: ribosylation ubiquitin; ubiquitin deltex; adp ribosylation; insights adp ... See more keywords
SUN-091 Functional Links between ADP-Ribosylation and Phosphorylation on Histone H2B Regulate Adipocyte Differentiation
Sign Up to like & getrecommendations! Published in 2019 at "Journal of the Endocrine Society"
DOI: 10.1210/js.2019-sun-091
Abstract: Abstract The differentiation of adipocyte precursors (preadipocytes) into mature adipocytes is a complex developmental process involving a carefully orchestrated transcriptional program, which is regulated by post-translational modification (PTM) of key target proteins. Our lab has… read more here.
Keywords: ribosylation; histone h2b; ribosylation phosphorylation; phosphorylation histone ... See more keywords
Protein and RNA ADP-ribosylation detection is influenced by sample preparation and reagents used
Sign Up to like & getrecommendations! Published in 2022 at "Life Science Alliance"
DOI: 10.26508/lsa.202201455
Abstract: Mono(ADP-ribosyl)ation is an emerging modification, for which recently detection reagents were generated. We cross-validated all reagents and observe remarkable differences in efficiency, depending on the substrate investigated and on the methods that are used. The… read more here.
Keywords: adp ribosylation; protein rna; sample preparation; detection ... See more keywords