Tractable Method for Rapid Quality Assessment of Therapeutic Antibodies in Harvested Cell Culture Fluid based on FcγRIIIa-Immobilized Magnetic Microspheres.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c01350
Abstract: FcγRIIIa-binding affinity is one of the key factors to ensure the efficacy of many antitumor therapeutic antibodies, which should be monitored along with the titer, protein aggregation, and other critical quality attributes. The conventional workflow… read more here.
Keywords: therapeutic antibodies; quality; quality assessment; riiia immobilized ... See more keywords
Biophysical Characterization of the Contribution of the Fab Region to the IgG-FcγRIIIa Interaction
Sign Up to like & getrecommendations! Published in 2022 at "Biochemistry"
DOI: 10.1021/acs.biochem.1c00832
Abstract: The cell-surface receptor FcγRIIIa is crucial to the efficacy of therapeutic antibodies as well as the immune response. The interaction of the Fc region of IgG molecules with FcγRIIIa has been characterized, but until recently,… read more here.
Keywords: riiia interaction; interaction; region igg; riiia ... See more keywords
Conformational effects of N-glycan core fucosylation of immunoglobulin G Fc region on its interaction with Fcγ receptor IIIa
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DOI: 10.1038/s41598-017-13845-8
Abstract: Antibody-dependent cellular cytotoxicity (ADCC) is promoted through interaction between the Fc region of immunoglobulin G1 (IgG1) and Fcγ receptor IIIa (FcγRIIIa), depending on N-glycosylation of these glycoproteins. In particular, core fucosylation of IgG1-Fc N-glycans negatively… read more here.
Keywords: igg1; core fucosylation; fucosylation; riiia ... See more keywords
Boosting of Markers of Fcγ Receptor Function in Anti-HIV Antibodies During Structured Treatment Interruption
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DOI: 10.1089/aid.2019.0047
Abstract: Abstract Anti-HIV envelope (Env) antibodies elicit important Fc receptor functions, including FcγRIIIa-mediated natural killer cell killing of opsonized infected targets. How these antibodies evolve during HIV infection and treatment remains poorly understood. We describe changes… read more here.
Keywords: anti hiv; structured treatment; riiia; anti ... See more keywords
Role of N-Glycosylation in FcγRIIIa interaction with IgG
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Immunology"
DOI: 10.3389/fimmu.2022.987151
Abstract: Immunoglobulins G (IgG) and their Fc gamma receptors (FcγRs) play important roles in our immune system. The conserved N-glycan in the Fc region of IgG1 impacts interaction of IgG with FcγRs and the resulting effector… read more here.
Keywords: interaction igg; glycosylation; role glycosylation; riiia ... See more keywords