PEG‐Like Brush Polymer Conjugate of RNA Aptamer That Shows Reversible Anticoagulant Activity and Minimal Immune Response
Sign Up to like & getrecommendations! Published in 2022 at "Advanced Materials"
DOI: 10.1002/adma.202107852
Abstract: Ribonucleic acid (RNA) therapeutics are an emerging class of drugs. RNA aptamers are of significant therapeutic and clinical interest because their activity can be easily reversed in vivo—a useful feature that is difficult to achieve… read more here.
Keywords: polymer; rna; rna aptamer; peg ... See more keywords
Development and Applications of Fluorogen/Light-Up RNA Aptamer Pairs for RNA Detection and More.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0712-1_5
Abstract: The central role of RNA in living systems made it highly desirable to have noninvasive and sensitive technologies allowing for imaging the synthesis and the location of these molecules in living cells. This need motivated… read more here.
Keywords: fluorogen light; light rna; rna aptamer; development applications ... See more keywords
Lighting-up RNA aptamer transcription synchronization amplification for ultrasensitive and label-free imaging of microRNA in single cells.
Sign Up to like & getrecommendations! Published in 2020 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2019.12.040
Abstract: Sensitive imaging of intracellular microRNAs (miRNAs) in cells is of great significance in clinical diagnoses and disease treatments, and it remains a major challenge to achieve this goal. Herein, we report a new in situ… read more here.
Keywords: lighting rna; rna aptamer; transcription synchronization; label free ... See more keywords
In silico designed RNA aptamer against epithelial cell adhesion molecule for cancer cell imaging.
Sign Up to like & getrecommendations! Published in 2019 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2019.01.028
Abstract: Aptamers are short, single-stranded oligonucleotides that bind to their targets with high affinity and specificity. Usually, aptamers are selected experimentally using SELEX approach. Here, we describe a computational approach for selection of aptamers for proteins,… read more here.
Keywords: epithelial cell; cell adhesion; rna aptamer; adhesion molecule ... See more keywords
Ligand specificity and affinity in the sulforhodamine B binding RNA aptamer.
Sign Up to like & getrecommendations! Published in 2020 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2020.06.056
Abstract: Binding affinity and selectivity are critical properties of aptamers that must be optimized for any application. The sulforhodamine B binding RNA aptamer (SRB-2) is a somewhat promiscuous aptamer that can bind ligands that vary markedly… read more here.
Keywords: binding rna; affinity; sulforhodamine binding; rna aptamer ... See more keywords
In vitro selection generates RNA aptamer that antagonizes PCSK9-LDLR interaction and recovers cellular LDL uptake.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of bioscience and bioengineering"
DOI: 10.1016/j.jbiosc.2020.10.009
Abstract: Proprotein convertase subtilisin/kexin type 9 (PCSK9) induces low-density lipoprotein (LDL)-receptor (LDLR) degradation, increasing plasma LDL-cholesterol levels and causing hypercholesterolemia. Therefore, inhibition of PCSK9-LDLR interaction is an attractive therapeutic target for hypercholesterolemia treatment. In this study,… read more here.
Keywords: rna aptamer; ldl uptake; ldlr interaction; pcsk9 ldlr ... See more keywords
Cascade Transcription Amplification of RNA Aptamer for Ultrasensitive MicroRNA Detection.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.9b00124
Abstract: MicroRNAs (miRNAs) play a critical role in multifarious biological processes and being deemed to be important biomarkers for clinical cancer diagnosis, prognosis, and therapy. Thus, assays for sensitive and accurate quantification of miRNAs are highly… read more here.
Keywords: detection; cascade transcription; rna aptamer; transcription amplification ... See more keywords
Real-Time Visualization of in Vitro Transcription of a Fluorescent RNA Aptamer: An Experiment for the Upper-Division Undergraduate or First-Year Graduate Laboratory
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Chemical Education"
DOI: 10.1021/acs.jchemed.7b00735
Abstract: A teaching laboratory experiment is described where students prepare in vitro transcription reactions of a fluorescent RNA aptamer, named Broccoli, and observe the production of the aptamer in real... read more here.
Keywords: fluorescent rna; vitro transcription; experiment; real time ... See more keywords
The First FRET-Based RNA Aptamer NanoKit for Sensitively and Specifically Detecting c-di-GMP.
Sign Up to like & getrecommendations! Published in 2022 at "Nano letters"
DOI: 10.1021/acs.nanolett.1c03970
Abstract: An effective method to identify c-di-GMP may significantly facilitate the exploration of its signaling pathways and bacterial pathogenesis. Herein, we have developed the first conjugated polymer-amplified RNA aptamer NanoKit with a unique core-shell-shell architecture, which… read more here.
Keywords: nanokit; detecting gmp; aptamer nanokit; rna aptamer ... See more keywords
Sampling Performance of Multiple Independent Molecular Dynamics Simulations of an RNA Aptamer
Sign Up to like & getrecommendations! Published in 2020 at "ACS Omega"
DOI: 10.1021/acsomega.0c01867
Abstract: Using multiple independent simulations instead of one long simulation has been shown to improve the sampling performance attained with the molecular dynamics (MD) simulation method. However, it is generally not known how long each independent… read more here.
Keywords: multiple independent; sampling performance; rna aptamer; novo predicted ... See more keywords
Subtle sequence variations alter tripartite complex kinetics and G-quadruplex dynamics in RNA aptamer Broccoli.
Sign Up to like & getrecommendations! Published in 2020 at "Chemical communications"
DOI: 10.1039/c9cc09375c
Abstract: Though extensively utilized, the fluorescent RNA aptamer Broccoli is poorly characterized with an unknown structure. Spectroscopic and kinetic investigations of tripartite complex formation reveal surprising differences between Broccoli and Spinach aptamers despite extreme sequence conservation.… read more here.
Keywords: subtle sequence; sequence variations; rna aptamer; tripartite complex ... See more keywords