Develop a Compact RNA Base Editor by Fusing ADAR with Engineered EcCas6e.
Sign Up to like & getrecommendations! Published in 2023 at "Advanced science"
DOI: 10.1002/advs.202206813
Abstract: Catalytically inactive CRISPR-Cas13 (dCas13)-based base editors can achieve the conversion of adenine-to-inosine (A-to-I) or cytidine-to-uridine (C-to-U) at the RNA level, however, the large size of dCas13 protein limits its in vivo applications. Here, a compact… read more here.
Keywords: compact rna; base editor; rna base; develop compact ... See more keywords
Programmable RNA editing with compact CRISPR–Cas13 systems from uncultivated microbes
Sign Up to like & getrecommendations! Published in 2021 at "Nature Methods"
DOI: 10.1038/s41592-021-01124-4
Abstract: Competitive coevolution between microbes and viruses has led to the diversification of CRISPR–Cas defense systems against infectious agents. By analyzing metagenomic terabase datasets, we identified two compact families (775 to 803 amino acids (aa)) of… read more here.
Keywords: rna base; rna editing; cas13; programmable rna ... See more keywords
Single-molecule RNA sequencing for simultaneous detection of m6A and 5mC
Sign Up to like & getrecommendations! Published in 2021 at "Scientific Reports"
DOI: 10.1038/s41598-021-98805-z
Abstract: Epitranscriptomics is the study of RNA base modifications involving functionally relevant changes to the transcriptome. In recent years, epitranscriptomics has been an active area of research. However, a major issue has been the development of… read more here.
Keywords: base modifications; single molecule; rna base;
Rescue of autosomal dominant hearing loss by in vivo delivery of mini dCas13X-derived RNA base editor
Sign Up to like & getrecommendations! Published in 2022 at "Science Translational Medicine"
DOI: 10.1126/scitranslmed.abn0449
Abstract: Programmable RNA editing tools enable the reversible correction of mutant transcripts, reducing the potential risk associated with permanent genetic changes associated with the use of DNA editing tools. However, the potential of these RNA tools… read more here.
Keywords: rna editing; hearing loss; base editor; rna base ... See more keywords
The complex, dynamic SpliceOme of the small GTPase transcripts altered by technique, sex, genetics, tissue specificity, and RNA base editing
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Cell and Developmental Biology"
DOI: 10.3389/fcell.2022.1033695
Abstract: The small GTPase family is well-studied in cancer and cellular physiology. With 162 annotated human genes, the family has a broad expression throughout cells of the body. Members of the family have multiple exons that… read more here.
Keywords: family; small gtpase; rna base; transcripts altered ... See more keywords
RNA Modification Related Diseases and Sensing Methods
Sign Up to like & getrecommendations! Published in 2023 at "Applied Sciences"
DOI: 10.3390/app13116376
Abstract: Epitranscriptomics is the study of RNA base modifications, including functionally relevant transcriptomic changes. Epitranscriptomics has been actively studied in recent years and has been reported to play important roles in development, homeostasis, the immune system,… read more here.
Keywords: modification; rna modification; base modifications; rna base ... See more keywords