Selection of RNA‐Cleaving TNA Enzymes for Cellular Mg2+ Imaging
Sign Up to like & getrecommendations! Published in 2022 at "ChemBioChem"
DOI: 10.1002/cbic.202200651
Abstract: Catalytic DNA‐based fluorescent sensors have enabled cellular imaging of metal ions such as Mg2+. However, natural DNA is prone to nuclease‐mediated degradation. Here, we report the in vitro selection of threose nucleic acid enzymes (TNAzymes) with… read more here.
Keywords: tna enzymes; rna cleaving; enzymes cellular; selection rna ... See more keywords
RNA-cleaving DNAzymes as a diagnostic and therapeutic agent against antimicrobial resistant bacteria.
Sign Up to like & getrecommendations! Published in 2021 at "Current genetics"
DOI: 10.1007/s00294-021-01212-0
Abstract: The development of nucleic-acid-based antimicrobials such as RNA-cleaving DNAzyme (RCD), a short catalytically active nucleic acid, is a promising alternative to the current antibiotics. The current rapid spread of antimicrobial resistance (AMR) in bacteria renders… read more here.
Keywords: therapeutic agent; agent; diagnostic therapeutic; dnazymes diagnostic ... See more keywords
Evolution of a General RNA-Cleaving FANA Enzyme
Sign Up to like & getrecommendations! Published in 2018 at "Nature Communications"
DOI: 10.1038/s41467-018-07611-1
Abstract: The isolation of synthetic genetic polymers (XNAs) with catalytic activity demonstrates that catalysis is not limited to natural biopolymers, but it remains unknown whether such systems can achieve robust catalysis with Michaelis-Menten kinetics. Here, we… read more here.
Keywords: rna; general rna; evolution general; rna cleaving ... See more keywords
An RNA-cleaving threose nucleic acid enzyme capable of single point mutation discrimination
Sign Up to like & getrecommendations! Published in 2021 at "Nature Chemistry"
DOI: 10.1038/s41557-021-00847-3
Abstract: Threose nucleic acid has been considered a potential evolutionary progenitor of RNA because of its chemical simplicity, base pairing properties and capacity for higher-order functions such as folding and specific ligand binding. Here we report… read more here.
Keywords: rna cleaving; threose nucleic; tz1; nucleic acid ... See more keywords
A Trisbenzimidazole Phosphoramidite Building Block Enables High-Yielding Syntheses of RNA-Cleaving Oligonucleotide Conjugates
Sign Up to like & getrecommendations! Published in 2020 at "Molecules"
DOI: 10.3390/molecules25081842
Abstract: The RNA cleaving catalyst tris(2-aminobenzimidazole) when attached to the 5’ terminus of oligonucleotides cuts complementary RNA strands in a highly site-specific manner. Conjugation was previously achieved by the acylation of an amino linker by an… read more here.
Keywords: trisbenzimidazole phosphoramidite; building block; phosphoramidite building; building ... See more keywords