Multivalent forms of the ribonuclease H1 hybrid binding domain are high‐affinity binders of RNA–DNA hybrids
Sign Up to like & getrecommendations! Published in 2022 at "FEBS Letters"
DOI: 10.1002/1873-3468.14541
Abstract: The hybrid binding domain (HBD) is a conserved fold present in ribonucleases H1 that selectively recognizes RNA–DNA hybrids, which are structures present in cellular R‐loops and participate in diverse biological processes. We engineered multivalent HBD… read more here.
Keywords: rna dna; hybrid binding; high affinity; affinity ... See more keywords
Assigning forensic body fluids to donors in mixed body fluids by targeted RNA/DNA deep sequencing of coding region SNPs
Sign Up to like & getrecommendations! Published in 2020 at "International Journal of Legal Medicine"
DOI: 10.1007/s00414-020-02252-w
Abstract: Biological traces found at crime scenes are analysed not only to genetically identify the donor(s) but also to determine the composition of the stain. For some cases, it is essential to associate a body fluid… read more here.
Keywords: body fluid; body fluids; rna dna; csnps ... See more keywords
Appraisal of Warm-Temperate South African Mangrove Estuaries as Habitats to Enhance Larval Nutritional Condition and Growth of Gilchristella aestuaria (Family Clupeidae) Using RNA:DNA Ratios
Sign Up to like & getrecommendations! Published in 2018 at "Estuaries and Coasts"
DOI: 10.1007/s12237-018-0375-x
Abstract: Estuaries are highly dynamic systems that serve as nursery areas to fishes and are likely to vary in nursery function, mostly due to habitat quality and food availability. Mangroves are thought to be good nurseries… read more here.
Keywords: nutritional condition; growth; mangrove estuaries; rna dna ... See more keywords
RNA/DNA ratio to assess the growth performance in postlarvae of the pearl oyster Pinctada fucata (Gould, 1850)
Sign Up to like & getrecommendations! Published in 2021 at "Aquaculture Reports"
DOI: 10.1016/j.aqrep.2021.100600
Abstract: Abstract RNA/DNA ratio (RDR) has been reported to be a feasible biochemical indicator for measuring the growth of numerous aquatic animal species. Nevertheless, its applicability has not been investigated to date in the pearl oyster… read more here.
Keywords: growth; pearl oyster; rna dna; oyster pinctada ... See more keywords
RNA/DNA Hybrid Interactome Identifies DXH9 as a Molecular Player in Transcriptional Termination and R-Loop-Associated DNA Damage
Sign Up to like & getrecommendations! Published in 2018 at "Cell Reports"
DOI: 10.1016/j.celrep.2018.04.025
Abstract: Summary R-loops comprise an RNA/DNA hybrid and displaced single-stranded DNA. They play important biological roles and are implicated in pathology. Even so, proteins recognizing these structures are largely undefined. Using affinity purification with the S9.6… read more here.
Keywords: dna; rna dna; dna hybrid; biology ... See more keywords
Phenoxazine nucleoside derivatives with a multiple activity against RNA and DNA viruses
Sign Up to like & getrecommendations! Published in 2021 at "European Journal of Medicinal Chemistry"
DOI: 10.1016/j.ejmech.2021.113467
Abstract: Emerging and re-emerging viruses periodically cause outbreaks and epidemics all over the world, eventually leading to global events such as the current pandemic of the novel SARS-CoV-2 coronavirus infection COVID-19. Therefore, an urgent need for… read more here.
Keywords: rna dna; rna; phenoxazine nucleoside; activity ... See more keywords
P251 Testing HIV and HBV viral load in ffpe transplant biopsies with the cobas ®mpliprep/cobas®taqman®system
Sign Up to like & getrecommendations! Published in 2017 at "Human Immunology"
DOI: 10.1016/j.humimm.2017.06.311
Abstract: Aim Determine if the COBAS® AmpliPrep/COBAS® TaqMan® System (COBAS® System) could be used to quantify viral loads of HIV and HBV in FFPE biopsies using as input sample purified RNA and DNA, respectively. Methods Cell… read more here.
Keywords: system; treated cells; dna; cobas ... See more keywords
Dual-channel sensing strategy based on gold nanoparticles cooperating with carbon dots and hairpin structure for assaying RNA and DNA.
Sign Up to like & getrecommendations! Published in 2017 at "Talanta"
DOI: 10.1016/j.talanta.2017.07.035
Abstract: By employing the attractive performance of fluorescent carbon dots and the assistant of hairpin structure, an innovative dual-channel biosensor on the basis of gold nanoparticles (AuNPs) for detecting multiple nucleotide sequences has been successfully proposed.… read more here.
Keywords: rna dna; carbon dots; hairpin structure; dna ... See more keywords
A diverse assemblage of RNA and DNA viruses found in mosquitoes collected in southern Portugal.
Sign Up to like & getrecommendations! Published in 2019 at "Virus research"
DOI: 10.1016/j.virusres.2019.197769
Abstract: This work describes the detection and partial characterization of mosquito-borne virus genomic sequences, based on the analysis of mosquitoes collected from the Spring to Fall of 2018 in the Algarve (southern Portugal). The viral survey… read more here.
Keywords: diverse assemblage; rna dna; assemblage rna; southern portugal ... See more keywords
The 2-Aminopyridine Nucleobase Improves Triple-Helical Recognition of RNA and DNA When Used Instead of Pseudoisocytosine in Peptide Nucleic Acids.
Sign Up to like & getrecommendations! Published in 2021 at "Biochemistry"
DOI: 10.1021/acs.biochem.1c00275
Abstract: Pseudoisocytosine (J), a neutral analogue of protonated cytosine, is currently the gold standard modified nucleobase in peptide nucleic acids (PNAs) for the formation of J·G-C triplets that are stable at physiological pH. This study shows… read more here.
Keywords: peptide nucleic; rna dna; triple helical; nucleic acids ... See more keywords
Bisubstrate Function of RNA Polymerases Triggered by Molecular Crowding Conditions.
Sign Up to like & getrecommendations! Published in 2019 at "Biochemistry"
DOI: 10.1021/acs.biochem.8b01204
Abstract: Since the origin of life on Earth, the role of carrying genetic information has been presumably transferred from RNA to DNA. At present, cellular environments are extremely dense, packed with cosolutes and macromolecules. Hence, the… read more here.
Keywords: dna; rna dna; rna; polymerase ... See more keywords