Addition-Elimination Mechanism-Activated Nucleotide Transition Sequencing for RNA Dynamics Profiling.
Sign Up to like & getrecommendations! Published in 2021 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.1c03361
Abstract: Dynamic information of intracellular transcripts is essential to understand their functional roles. Routine RNA-sequencing (RNA-seq) methods only measure RNA species at a steady state and do not provide RNA dynamic information. Here, we develop addition-elimination… read more here.
Keywords: rna dynamics; addition elimination; chemistry; transition ... See more keywords
RNA dynamics revealed by metabolic RNA labeling and biochemical nucleoside conversions
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DOI: 10.1038/nmeth.4608
Abstract: The combination of metabolic RNA labeling with biochemical nucleoside conversion now adds a broadly applicable temporal dimension to RNA sequencing. read more here.
Keywords: rna dynamics; metabolic rna; biochemical nucleoside; rna ... See more keywords
Evolution of protein-coupled RNA dynamics during hierarchical assembly of ribosomal complexes
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DOI: 10.1038/s41467-017-00536-1
Abstract: Assembly of 30S ribosomes involves the hierarchical addition of ribosomal proteins that progressively stabilize the folded 16S rRNA. Here, we use three-color single molecule FRET to show how combinations of ribosomal proteins uS4, uS17 and… read more here.
Keywords: protein; rna dynamics; protein coupled; bs16 binds ... See more keywords
Ribosome-dependent conformational flexibility changes and RNA dynamics of IRES domains revealed by differential SHAPE
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DOI: 10.1038/s41598-018-23845-x
Abstract: Internal ribosome entry site (IRES) elements are RNA regions that recruit the translation machinery internally. Here we investigated the conformational changes and RNA dynamics of a picornavirus IRES upon incubation with distinct ribosomal fractions. Differential… read more here.
Keywords: rna dynamics; differential shape; ribosome dependent; flexibility ... See more keywords