Quantification of MicroRNAs in human aqueous humor by miRFLP assay
Sign Up to like & getrecommendations! Published in 2017 at "Experimental Eye Research"
DOI: 10.1016/j.exer.2017.07.007
Abstract: ABSTRACT MiRNAs are expressed in a tissueâspecific fashion in the eyes and changes in miRNAs levels in aqueous humor (AH) may reflect the function of the eye and eye disease. Due to the low concentration… read more here.
Keywords: rna extraction; mirflp assay; aqueous humor; human aqueous ... See more keywords
Evaluating the performance of five up-to-date DNA/RNA co-extraction methods for forensic application.
Sign Up to like & getrecommendations! Published in 2021 at "Forensic science international"
DOI: 10.1016/j.forsciint.2021.110996
Abstract: The importance of RNA evidence is growing with new developments in RNA profiling methods and purposes. As forensic samples often can be of small quantity, extraction methods with high yields of both DNA and RNA… read more here.
Keywords: extraction; rna extraction; extraction methods; rna ... See more keywords
Standardization of an efficient protocol for isolation of RNA from Cuminum cyminum
Sign Up to like & getrecommendations! Published in 2019 at "Journal of King Saud University - Science"
DOI: 10.1016/j.jksus.2018.12.008
Abstract: Abstract Cumin is one of the oldest seed species and second most popular after black pepper. Till date molecular work into cumin has been principally related to studying diversity based on phenotypic, biochemical and molecular… read more here.
Keywords: rna extraction; quality rna; quality; method ... See more keywords
Sample preservation, transport and processing strategies for honeybee RNA extraction: Influence on RNA yield, quality, target quantification and data normalization.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of virological methods"
DOI: 10.1016/j.jviromet.2017.04.010
Abstract: Viral infections in managed honey bees are numerous, and most of them are caused by viruses with an RNA genome. Since RNA degrades rapidly, appropriate sample management and RNA extraction methods are imperative to get… read more here.
Keywords: extraction; quality; rna extraction; rna yield ... See more keywords
Protocol optimization for simultaneous DNA and RNA co-extraction from single hard tick specimens
Sign Up to like & getrecommendations! Published in 2021 at "MethodsX"
DOI: 10.1016/j.mex.2021.101315
Abstract: Hard ticks are important vectors of DNA- and RNA-based infectious microorganisms, but they also host complex microbial communities in which pathogens and symbionts can interact among each other and with the arthropod host itself. Molecular… read more here.
Keywords: rna extraction; dna rna; hard tick; dna ... See more keywords
RNA-extraction-free diagnostic method to detect SARS-CoV-2: an assessment from two states, India
Sign Up to like & getrecommendations! Published in 2021 at "Epidemiology and Infection"
DOI: 10.1017/s0950268821002302
Abstract: Abstract With increasing demand for large numbers of testing during the coronavirus disease 2019 pandemic, alternative protocols were developed with shortened turn-around time. We evaluated the performance of such a protocol wherein 1138 consecutive clinic… read more here.
Keywords: rna extraction; reference; method; index method ... See more keywords
Improved Reversible Cross-Linking-Based Solid-Phase RNA Extraction for Pathogen Diagnostics.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.7b03493
Abstract: In this study, we developed an amine-functionalized, diatomaceous earth-based, dimethyl suberimidate assisted (ADD) system as a novel binding strategy to improve the solid-phase extraction method for rapid and simple purification of RNA from biological samples… read more here.
Keywords: reversible cross; extraction; rna extraction; solid phase ... See more keywords
RNA-extraction-free nano-amplified colorimetric test for point-of-care clinical diagnosis of COVID-19.
Sign Up to like & getrecommendations! Published in 2021 at "Nature protocols"
DOI: 10.1038/s41596-021-00546-w
Abstract: The global pandemic of coronavirus disease 2019 (COVID-19) highlights the shortcomings of the current testing paradigm for viral disease diagnostics. Here, we report a stepwise protocol for an RNA-extraction-free nano-amplified colorimetric test for rapid and… read more here.
Keywords: rna extraction; nano amplified; free nano; extraction free ... See more keywords
Complete sequence-based pathway analysis by differential on-chip DNA and RNA extraction from a single cell
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-10704-4
Abstract: We demonstrate on-chip, differential DNA and RNA extraction from a single cell using a microfluidic chip and a two-stage lysis protocol. This method enables direct use of the whole extract, without additional washing steps, reducing… read more here.
Keywords: single cell; rna extraction; dna rna; extraction single ... See more keywords
Optimized and scalable synthesis of magnetic nanoparticles for RNA extraction in response to developing countries' needs in the detection and control of SARS-CoV-2
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-75798-9
Abstract: Ecuador is one of the most affected countries, with the coronavirus disease 2019 (COVID-19) infection, in Latin America derived from an ongoing economic crisis. One of the most important methods for COVID-19 detection is the… read more here.
Keywords: extraction; detection; rna extraction; optimized scalable ... See more keywords
Total RNA Extraction from Saccharomyces cerevisiae Using Hot Acid Phenol.
Sign Up to like & getrecommendations! Published in 2021 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot101691
Abstract: Isolation of RNA from yeast is complicated by the need to first break the thick, rigid cell wall. The protocol provided here uses a cycle of heating and freezing of cells in the presence of… read more here.
Keywords: extraction; saccharomyces cerevisiae; phenol; total rna ... See more keywords