Preparation of Total RNA from Fission Yeast.
Sign Up to like & getrecommendations! Published in 2017 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot091629
Abstract: Treatment with hot phenol breaks open fission yeast cells and begins to strip away bound proteins from RNA. Deproteinization is completed by multiple extractions with chloroform/isoamyl alcohol and separation of the aqueous and organic phases… read more here.
Keywords: rna fission; fission; total rna; fission yeast ... See more keywords