The Manipulation of RNA‐Guided Nucleic Acid Cleavage with Ninhydrin Chemistry
Sign Up to like & getrecommendations! Published in 2020 at "Advanced Science"
DOI: 10.1002/advs.201903770
Abstract: Abstract CRISPR (clustered regularly interspaced short palindromic repeats) systems have been established as valuable genome‐editing tools. Controlling CRISPR systems has high biological significance and this field has garnered intense interest. There is a considerable need… read more here.
Keywords: crispr systems; ninhydrin chemistry; rna guided; chemistry ... See more keywords
Structural Basis for the RNA-Guided Ribonuclease Activity of CRISPR-Cas13d
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DOI: 10.1016/j.cell.2018.09.001
Abstract: CRISPR-Cas endonucleases directed against foreign nucleic acids mediate prokaryotic adaptive immunity and have been tailored for broad genetic engineering applications. Type VI-D CRISPR systems contain the smallest known family of single effector Cas enzymes, and… read more here.
Keywords: structural basis; rna guided; ribonuclease; rna ... See more keywords
Transposons and CRISPR: Rewiring Gene Editing.
Sign Up to like & getrecommendations! Published in 2022 at "Biochemistry"
DOI: 10.1021/acs.biochem.2c00379
Abstract: CRISPR-Cas is driving a gene editing revolution because of its simple reprogramming. However, off-target effects and dependence on the double-strand break repair pathways impose important limitations. Because homology-directed repair acts primarily in actively dividing cells,… read more here.
Keywords: crispr rewiring; gene editing; transposons crispr; rna guided ... See more keywords
Transposon-encoded CRISPR–Cas systems direct RNA-guided DNA integration
Sign Up to like & getrecommendations! Published in 2019 at "Nature"
DOI: 10.1038/s41586-019-1323-z
Abstract: Conventional CRISPR–Cas systems maintain genomic integrity by leveraging guide RNAs for the nuclease-dependent degradation of mobile genetic elements, including plasmids and viruses. Here we describe a notable inversion of this paradigm, in which bacterial Tn7-like… read more here.
Keywords: crispr cas; cas systems; rna guided; dna ... See more keywords
Therapeutic applications of CRISPR RNA-guided genome editing.
Sign Up to like & getrecommendations! Published in 2017 at "Briefings in functional genomics"
DOI: 10.1093/bfgp/elw032
Abstract: The rapid development of programmable nuclease-based genome editing technologies has enabled targeted gene disruption and correction both in vitro and in vivo This revolution opens up the possibility of precise genome editing at target genomic… read more here.
Keywords: genome editing; crispr rna; therapeutic applications; rna guided ... See more keywords
RNA-guided DNA base damage repair via DNA polymerase-mediated nick translation
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac1178
Abstract: Abstract DNA repair is mediated by DNA synthesis guided by a DNA template. Recent studies have shown that DNA repair can also be accomplished by RNA-guided DNA synthesis. However, it remains unknown how RNA can… read more here.
Keywords: dna; damage; guided dna; rna guided ... See more keywords
Functional characterization of diverse type I-F CRISPR-associated transposons
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac985
Abstract: Abstract CRISPR-Cas systems generally provide adaptive immunity in prokaryotes through RNA-guided degradation of foreign genetic elements like bacteriophages and plasmids. Recently, however, transposon-encoded and nuclease-deficient CRISPR-Cas systems were characterized and shown to be co-opted by… read more here.
Keywords: crispr; type crispr; rna guided; crispr cas ... See more keywords
Novel molecular requirements for CRISPR RNA-guided transposition
Sign Up to like & getrecommendations! Published in 2023 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkad270
Abstract: Abstract CRISPR-associated transposases (CASTs) direct DNA integration downstream of target sites using the RNA-guided DNA binding activity of nuclease-deficient CRISPR-Cas systems. Transposition relies on several key protein-protein and protein-DNA interactions, but little is known about… read more here.
Keywords: dna; molecular requirements; integration; transposition ... See more keywords
RNA-guided DNA insertion with CRISPR-associated transposases
Sign Up to like & getrecommendations! Published in 2019 at "Science"
DOI: 10.1126/science.aax9181
Abstract: Beyond adaptive immunity Prokaryotic CRISPR-Cas systems defend bacterial cells from phage and plasmid infection. Strecker et al. characterized a CRISPR-Cas system that functions beyond adaptive immunity (see the Perspective by Hou and Zhang). Type V-K… read more here.
Keywords: crispr; dna; insertion; rna guided ... See more keywords
Craspase is a CRISPR RNA-guided, RNA-activated protease
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DOI: 10.1126/science.add5064
Abstract: The CRISPR-Cas type III-E RNA-targeting effector complex gRAMP/Cas7-11 is associated with a caspase-like protein (TPR-CHAT/Csx29) to form Craspase (CRISPR-guided caspase). Here, we use cryo–electron microscopy snapshots of Craspase to explain its target RNA cleavage and… read more here.
Keywords: protease; crispr; craspase; rna ... See more keywords
RNA-activated protein cleavage with a CRISPR-associated endopeptidase
Sign Up to like & getrecommendations! Published in 2022 at "Science"
DOI: 10.1126/science.add7450
Abstract: In prokaryotes, CRISPR-Cas systems provide adaptive immune responses against foreign genetic elements through RNA-guided nuclease activity. Recently, additional genes with non-nuclease functions have been found in genetic association with CRISPR systems, suggesting that there may… read more here.
Keywords: rna activated; rna guided; crispr; activated protein ... See more keywords