RNA Imaging with RNase-Inactivated Csy4 in Plants and Filamentous Fungi.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0712-1_9
Abstract: Subcellular localizations of RNAs can be imaged in vivo with genetically encoded reporters consisting of a sequence-specific RNA-binding protein (RBP) fused to a fluorescent protein. Several such reporter systems have been described based on RBPs… read more here.
Keywords: plants filamentous; csy4 plants; inactivated csy4; rnase inactivated ... See more keywords
Live-Cell Single RNA Imaging Reveals Bursts of Translational Frameshifting.
Sign Up to like & getrecommendations! Published in 2019 at "Molecular cell"
DOI: 10.1016/j.molcel.2019.05.002
Abstract: Ribosomal frameshifting during the translation of RNA is implicated in human disease and viral infection. While previous work has uncovered many details about single RNA frameshifting kinetics in vitro, little is known about how single RNA… read more here.
Keywords: live cell; rna; cell single; single rna ... See more keywords
Cascade Circuits on Self-Assembled DNA Polymers for Targeted RNA Imaging In Vivo.
Sign Up to like & getrecommendations! Published in 2020 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.0c03400
Abstract: DNA molecular probes have emerged as a powerful tool for RNA imaging. Hurdles in cell-specific delivery and other issues such as insufficient stability, limited sensitivity, or slow reaction kinetics, however, hinder the further application of… read more here.
Keywords: dna; imaging vivo; reaction; dna polymers ... See more keywords
SiRA: A Silicon Rhodamine-Binding Aptamer for Live-Cell Super-Resolution RNA Imaging.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.9b02697
Abstract: Although genetically encoded light-up RNA aptamers have become promising tools for visualizing and tracking RNAs in living cells, aptamer/ligand pairs that emit in the far-red and near-infrared (NIR) regions are still rare. In this work,… read more here.
Keywords: rna imaging; live cell; super resolution; rna ... See more keywords
Super-resolution RNA imaging using a rhodamine-binding aptamer with fast exchange kinetics.
Sign Up to like & getrecommendations! Published in 2021 at "Nature biotechnology"
DOI: 10.1038/s41587-020-00794-3
Abstract: Overcoming limitations of previous fluorescent light-up RNA aptamers for super-resolution imaging, we present RhoBAST, an aptamer that binds a fluorogenic rhodamine dye with fast association and dissociation kinetics. Its intermittent fluorescence emission enables single-molecule localization… read more here.
Keywords: resolution; resolution rna; super resolution; rna imaging ... See more keywords
Highly specific multiplexed RNA imaging in tissues with split-FISH
Sign Up to like & getrecommendations! Published in 2020 at "Nature Methods"
DOI: 10.1038/s41592-020-0858-0
Abstract: We present split-FISH, a multiplexed fluorescence in situ hybridization method that leverages a split-probe design to achieve enhanced specificity. Split-FISH reduces off-target background fluorescence, decreases false positives and enables accurate RNA profiling in uncleared tissues.… read more here.
Keywords: multiplexed rna; highly specific; tissues split; specific multiplexed ... See more keywords
Recent advances in high-performance fluorescent and bioluminescent RNA imaging probes.
Sign Up to like & getrecommendations! Published in 2017 at "Chemical Society reviews"
DOI: 10.1039/c6cs00675b
Abstract: RNA plays an important role in life processes. Imaging of messenger RNAs (mRNAs) and micro-RNAs (miRNAs) not only allows us to learn the formation and transcription of mRNAs and the biogenesis of miRNAs involved in… read more here.
Keywords: imaging probes; life processes; rna; rna imaging ... See more keywords
Live-cell RNA imaging using the CRISPR-dCas13 system with modified sgRNAs appended with fluorescent RNA aptamers
Sign Up to like & getrecommendations! Published in 2022 at "Chemical Science"
DOI: 10.1039/d2sc04656c
Abstract: The development of RNA imaging strategies in live cells is essential to improve our understanding of their role in various cellular functions. We report an efficient RNA imaging method based on the CRISPR-dPspCas13b system with… read more here.
Keywords: system; crispr; rna imaging; rna ... See more keywords
Illuminating Genomic Dark Matter with RNA Imaging.
Sign Up to like & getrecommendations! Published in 2019 at "Cold Spring Harbor perspectives in biology"
DOI: 10.1101/cshperspect.a032094
Abstract: SUMMARYIn the postgenomic era, it is clear that the human genome encodes thousands of long noncoding RNAs (lncRNAs). Along the way, RNA imaging (e.g., RNA fluorescence in situ hybridization [RNA-FISH]) has been instrumental in identifying… read more here.
Keywords: dark matter; biology; illuminating genomic; rna imaging ... See more keywords
A CRISPR-Cas and Tat Peptide with Fluorescent RNA Aptamer System for Signal Amplification in RNA Imaging
Sign Up to like & getrecommendations! Published in 2023 at "Biosensors"
DOI: 10.3390/bios13020293
Abstract: We reported on an efficient RNA imaging strategy based on a CRISPR-Cas and Tat peptide with a fluorescent RNA aptamer (TRAP-tag). Using modified CRISPR-Cas RNA hairpin binding proteins fused with a Tat peptide array that… read more here.
Keywords: peptide fluorescent; rna imaging; rna; cas tat ... See more keywords