Bias in recent miRBase annotations potentially associated with RNA quality issues
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-05070-0
Abstract: Although microRNAs are supposed to be stable in-vivo, degradation processes potentially blur our knowledge on the small oligonucleotides. We set to quantify the effect of degradation on microRNAs in mouse to identify causes for distorted… read more here.
Keywords: degradation; mirbase; recent mirbase; bias recent ... See more keywords
Influence of Freeze-Thaw Cycles on RNA Integrity of Gastrointestinal Cancer and Matched Adjacent Tissues.
Sign Up to like & getrecommendations! Published in 2017 at "Biopreservation and biobanking"
DOI: 10.1089/bio.2016.0035
Abstract: BACKGROUND Comparative analysis of RNA expression profiles between cancer and adjacent noncancerous tissues is an important part of cancer research. High-quality RNA is essential for consistent, reliable results, especially for identification of cancer biomarkers. However,… read more here.
Keywords: cancer; rna integrity; gastrointestinal cancer; freeze thaw ... See more keywords
Banking of Tumor Tissues: Effect of Preanalytical Variables in the Phase of Pre- and Postacquisition on RNA Integrity.
Sign Up to like & getrecommendations! Published in 2022 at "Biopreservation and biobanking"
DOI: 10.1089/bio.2021.0124
Abstract: Background: RNA integrity of tumor tissues from 12 common organs was measured, and tumor tissues from liver were found to have the best RNA integrity in our previous study. The effects of preanalytical variables in… read more here.
Keywords: rna integrity; preanalytical variables; tumor; tumor tissues ... See more keywords
Differential ratio amplicons (R amp) for the evaluation of RNA integrity extracted from complex environmental samples
Sign Up to like & getrecommendations! Published in 2019 at "Environmental Microbiology"
DOI: 10.1111/1462-2920.14516
Abstract: Summary Reliability and reproducibility of transcriptomicsâbased studies are dependent on RNA integrity. In microbial ecology, microfluidicsâbased techniques, such as the Ribosomal Integrity Number (RIN), targeting rRNA are currently the only approaches to evaluate RNA integrity.… read more here.
Keywords: rna; complex environmental; ratio; integrity ... See more keywords
Blood RNA Integrity is a Direct and Simple Reporter of Radiation Exposure and Prognosis: A Pilot Study
Sign Up to like & getrecommendations! Published in 2020 at "Radiation Research"
DOI: 10.1667/rr15527.1
Abstract: In the event of a mass casualty radiation scenario, rapid assessment of patients' health and triage is required for optimal resource utilization. Identifying the level and extent of exposure as well as prioritization of care… read more here.
Keywords: rna integrity; blood; radiation; exposure ... See more keywords