A versatile tandem RNA isolation procedure to capture in vivo formed mRNA-protein complexes.
Sign Up to like & getrecommendations! Published in 2017 at "Methods"
DOI: 10.1016/j.ymeth.2016.10.005
Abstract: We describe a tandem RNA isolation procedure (TRIP) that enables purification of in vivo formed messenger ribonucleoprotein (mRNP) complexes. The procedure relies on the purification of polyadenylated mRNAs with oligo(dT) beads from cellular extracts, followed… read more here.
Keywords: vivo; rna; rna isolation; procedure ... See more keywords
Total RNA Isolation from Drosophila melanogaster.
Sign Up to like & getrecommendations! Published in 2020 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot101675
Abstract: Of the methods available for extracting total RNA from Drosophila, monophasic lysis reagents (such as TRIzol) yield consistent and reliable results and are considerably cheaper than other kit-based techniques for RNA isolation. In this protocol,… read more here.
Keywords: drosophila; rna isolation; isolation drosophila; drosophila melanogaster ... See more keywords
Comparison of RNA isolation and library preparation methods for small RNA sequencing of canine biofluids.
Sign Up to like & getrecommendations! Published in 2019 at "Veterinary clinical pathology"
DOI: 10.1111/vcp.12743
Abstract: BACKGROUND Small RNA sequencing (RNA-seq) of biofluids is challenging due to the relative scarcity of microRNAs (miRNAs), limited sample volumes, and the lack of a gold standard isolation method. Additionally, few comparisons exist for the… read more here.
Keywords: small rna; preparation methods; library preparation; rna isolation ... See more keywords
Comparison of RNA isolation methods on RNA-Seq: implications for differential expression and meta-analyses
Sign Up to like & getrecommendations! Published in 2020 at "BMC Genomics"
DOI: 10.1186/s12864-020-6673-2
Abstract: Background The increasing number of transcriptomic datasets has allowed for meta-analyses, which can be valuable due to their increased statistical power. However, meta-analyses can be confounded by so-called “batch effects,” where technical variation across different… read more here.
Keywords: meta analyses; isolation; differential expression; rna isolation ... See more keywords